MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

MOLECULAR DETECTION AND PHYLOGENETIC ANALYSIS OF CUTANEOUS LEISHMANIASIS ISOLATED FROM STRAY DOGS SKIN IN RIYADH PROVINCE, SAUDI ARABIA

Type
Oral Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Neglected Tropical Diseases

Authors

Main Author
Abdullah Alanazi1
Presenting Author
Abdullah Alanazi1
Co-Author
Rahi Abdulsadah2
Magda Ali2
Mohamed Alyousif3
Ibrahim Alanazi 4
Sobhy Abdel-Shafy 5
Mona Mahmoud5

Authors' Institution

Department / Institution / Country
Department of Biological Sciences / Shaqra University / Saudi Arabia (المملكة العربية السعودية)1
College of Science / University of Wasit / Iraq (العراق)2
Department of Zoology / King Saud University / Saudi Arabia (المملكة العربية السعودية)3
The National Center for Biotechnology Technology / King Abdulaziz City for Science and technology / Saudi Arabia (المملكة العربية السعودية)4
Department of Parasitology and Animal Diseases / National Research Centre / Egypt, Arab Rep.5
Content
Abstract Content

Cutaneous leishmaniasis (CL) is caused by infection with protozoan parasites belonging to the genus Leishmania. It is transmitted through the bite of infected sand flies species of genus  Phlebotomus or Lutzomyia . Since dogs can be infected by sand flies in endemic areas, infected stray dogs may be a mobile risk for nonendemic areas.Therfore, the aims of this study were to determine the prevalence of canine leishmaniasis using nested PCR (nPCR) among stray dogs living in different districts of Riyadh Province, Saudi Arabia, where the disease is endemic and to identify and document different Leishmania to species levels. This cross-sectional investigation was conducted over the course of two years, from March 2016 to July 2018, in different districts of Riyadh Province, Saudi Arabia. A total of 237 dogs; 18 of the dogs were suspected clinically of cutaneous leishmaniasis due to the presence of cutaneous nodules. Biopsy tissue collections were performed and DNA was extracted. CSB2XF and CSB1XR primers were used to amplify the Leishmania kDNA regions. The Leishmania species were detected by specific 13Z and LIR primers by applying nested PCR (nPCR) assay.

Results and Discussion: Nine dogs were found to be positive for L.major. The examined dogs were negative for other Leishmania sp. The phylogenetic data and blast results of kDNA showed that the  9 isolates L. major is closely related  (99.9%) to the L. major isolate CMG_irfan5, accession number HQ727556.1 from human, Pakistan. The sequences of the 9 L. major isolates were deposited in GenBank.  In this study, the cutaneous leishmaniasis was found in the rural areas. It was shown that 99.99 % of isolate samples were caused by L. major, indicating the presence of a definite relationship between the disease vectors and reservoirs with transmission cycle elements in this area.

Keywords: L. major ; L. tropica ; Dogs ; kDNA ; nPCR ; Saudi Arabia
Requires Audio or Video system for Presentation?: No