MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur



Prevalence of Clostridium difficile infection in hospitalized adult patients from southwest region of Saudi Arabia

Oral Presentation
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Medical Microbiology & Parasitology


Main Author
Ahmad Hassan Ahmad Ghzwani1
Presenting Author
Ahmad Hassan Ahmad Ghzwani1
Khalid Ammash Mohammed Zain1
Ahmed Abdulhaq1
Mohammed Uthman A. Alamoudi1
Vinod Kumar Basode1

Authors' Institution

Department / Institution / Country
Unit of Medical Microbiology, Department of Medical Lab Technology, Faculty of Applied ‎Medical Scie / Jazan University / Saudi Arabia (المملكة العربية السعودية)1
Abstract Content

Clostridium difficile is a spore-forming, gram-positive, anaerobic bacillus, which is a frequent cause of antibiotic-associated diarrhoea, especially amongst hospitalized patients. This organism may cause antibiotic associated diarrhoea, occasionally leading to a life-threatening condition and pseudomembranous colitis. C. difficile is known as the most common hospital acquired infection in the patients in western countries, with limited number of studies from developing countries including Saudi Arabia. Apart from this there is no study from Jazan province, Saudi Arabia; hence, the present study was carried out. A two-step testing algorithm for detection of toxigenic C. difficile in stool, based on initial screening of stool samples by using certest C. difficile GDH+Toxin A+B (Cer Test, Spain) and confirmed by molecular analysis by using GeneXpert C. difficile polymerase chain reaction (PCR) assay (Cepheid, CA, USA) that detects the toxin B gene (tcdB), the binary toxin gene (cdt) and tcdC gene was carried out. There are about 7.40% of samples were positive by immunological method and 4.62% were positive by PCR technique out of 108 total samples collected. Understanding of the prevalence and molecular characterization of C. difficile will provide appropriate therapeutic decisions and also help to control infection from C. difficile. This study will provide a clear and comprehensive picture on the current status of Clostridium difficile in Jazan province, Saudi Arabia.

Requires Audio or Video system for Presentation?: Yes