MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur



Genetic analyses of rifampicin and streptomycin resistant mycobacterium tuberculosis strain isolated in Sabah, Malaysia

Oral Presentation
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Medical Microbiology & Parasitology


Main Author
Kamruddin Ahmed1
Presenting Author
Kamruddin Ahmed1
Jaeyres Jani1
Zainal Arifin Mustapha1
Kai Ling Chin1
Richard Avoi1
Oo Tha Naing1
Twee Hee Rick Ong1
Valentine Gantul1
Zainal Arifin Mustapha1

Authors' Institution

Department / Institution / Country
Borneo Medical and Health Research Centre / Faculty of Medicine and Health Sciences / Universiti Malaysia Sabah / Malaysia1
Abstract Content
Abstract Content: Tuberculosis (TB) is one of the top 10 causes of death worldwide. An estimated 10.4 million people are infected with TB, and 1.7 million die from the disease. The situation is becoming disturbing with multi-drug resistant TB (MDR-TB). In 2016, an estimated 490,000 people worldwide developed MDR-TB and an additional 110,000 people with rifampicin-resistant TB. In South East Asia, Malaysia is considered to be alow burden TB country. In 2016, 25,747 people were infected with TB which was 6.3% higher than in 2015, indicating an increasing trend of TB in Malaysia. In 2017, 370 cases of MDR-TB were reported in Malaysia of which 8 cases were from Sabah. In Malaysia, Sabah is one of the states with high prevalence of TB. In 2017, Sabah contributed 19.5% (5106) of total cases of TB in Malaysia. The reasons for the high TB burden in Sabah are not clearly understood, including the antibiotic resistance in treatment failure cases. In this study, we performed a whole-genome sequence analysis of Mycobacterium tuberculosis(MTB) strain,  isolated from sputum of a patient with TB to determine the lineage distribution and mutation site of antibiotic resistance. The sample was diagnosed as positive for rifampicin resistant MTB by GeneXpert.The de novo assembly of genome produced 135 contigs withN50 of 97,881 bp. The GC content is 65.55% and consists of 4,143 predicted genes. Phylogenetic analysis using the neighbor-joining method showed that our strain belongs to Lineage 1. Based on the variance calling analysis through the Genome Analysis Toolkit (GATK) tools, point mutation K43R was determined in the rpsL gene which confers resistance to streptomycin. Another mutation was detected at A1075 in rpoB gene which confers resistance to rifampicin.
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