MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur



Susceptibility Status of Aedes albopictus (Skuse) and Aedes aegypti (L.) Post-Outdoor Residual Spray (ORS) Treatment in Johor Bahru, Malaysia

E-Poster Presentation
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Vector Biology & Control


Main Author
Ainaa Mardiaa Bachtiar Effendi1
Presenting Author
Ainaa Mardiaa Bachtiar Effendi1
Nurulhusna Ab Hamid1
Fatin Athirah Azman1
Rohaiyu Md Rodzay1
Muhammad Khairi Kamaruzaman1
Topek Omar2
Rahmat Dapari2
Shahrom Nor Azian Che Mat3
Roslinda Abdul Rahman3
Ropiah Jara'ee
Mitra Saadatian-Elahi4
Nur Rasyidah Isa
Afiq Ahnaf Hafisol
Balqis Md Ruslan
Siti Farah Abdulah
Siti Fadhlina Mohd Noor

Authors' Institution

Department / Institution / Country
Medical Entomology Unit / Institute For Medical Research / Malaysia1
Vector Borne Disease Sector, Disease Control Division / Ministry of Health Malaysia / Malaysia2
Public Health Division / Johor State Health Department / Malaysia3
Service d’Hygiène, Epidémiologie et Prévention / Hospices Civils de Lyon / France4
Abstract Content
Outdoor Residual Spray (ORS) is one of the effective vector control methods which aims at reducing the infectious Aedes vectors from transmitting dengue virus to human. ORS involves the application and deposition of insecticides on the outer walls of premises to target Aedes spp. that will be exposed to the lethal dose while resting on the walls. To determine the susceptibility status of Aedes spp. pre - and post - ORS application, field strains were collected using ovitraps from four different localities, namely Flat Suria Muafakat (FSM), Taman Kempas Permai (TKP), Apartment Sri Wangi (ASW) and Pangsapuri Sri Indah (PSI) in Johor Bahru. These field strains were bred until F4 generations which were used to evaluate their resistance status in accordance to WHO guidelines. The mosquitoes are susceptible to the test insecticides if the mortality rate is in the range of 98-100% and is resistant if <98%. Adult bioassay results showed that post-ORS treatment Ae.albopictus was susceptible to the pyrethroid deltamethrin 0.03% and permethrin 0.25% for all localities, with mortality rates >98%, comparable to pre - ORS treatment which was <96%,except for ASW. In contrast, Ae.aegypti exhibited resistance against both pyrethroid insecticides with mortality rates <90% at pre - and post - ORS treatment. In addition, both Aedesspp. at all localities were resistant to the organophosphate malathion 0.8% with mortality rates <73% atpre- and post-ORS treatment. Although resistance to pyrethroids was found in Ae aegypti, nevertheless, control failure with the use of the application dosages was not observed.
Keywords: Aedes, Outdoor Residual Spray, Vector Control, Dengue,
Requires Audio or Video system for Presentation?: No