MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

Centella asiatica protects against cognitive deficits induced by D-galactose and aluminium chloride by attenuating oxidative stress and acetylcholinesterase activity

Type
Oral Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
General Topics

Authors

Main Author
Musa Samaila Chiroma1 2
Presenting Author
Musa Samaila Chiroma1 2
Co-Author
Mohamad Aris Mohd Moklas2
Mohamad Taufik Hidayat Baharuldin2
Che Norma Mat Taib2
Zulkhairi Amom5
Saravanan Jagadeesan2 3
Onesimus Mahdi2 4

Authors' Institution

Department / Institution / Country
Human Anatomy / University of Maiduguri / Nigeria1
Human Anatomy / Universiti Putra Malaysia / Malaysia2
Human Anatomy / UTAR Sungai Long / Malaysia3
Human Anatomy / Gombe State University / Nigeria4
Physiology / UiTM Puncak Alam / Malaysia5
Content
Abstract Content

Alzheimer’s disease (AD) is a serious multifactorial disorder with progressive neurodenerative outcomes related with cholinergic dysfunction and impaired redox homeostasis. The plant Centella asiatica (CA) is renowned for its nutritional benefits and herbal formulas to promote health, enhance cognition and neuroprotective abilities. The present study aimed to investigate the protective role of CA on D-gal and AlCl3 induced neurotoxicity and cognitive impairment on rats. Rats were divided into six groups and exposed to donepezil 1 mg/kg/day, CA (200, 400 and 800 mg/kg/day) and D-gal mg/kg/day + AlCl3 200 mg/kg/day for 10 weeks. The ethology of the rats were evaluated by Morris water maze test. The content of acetylcholinesterase (AChE), phosphorylated (P-tau), malondialdehyde (MDA) and superoxide dismutase (SOD) in the hippocampus and cerebral cortex were detected by enzyme-linked immunosorbent assay. Additionally, ultrastructure of the rats’ cerebral cortex was evaluated using transmission electron microscopy (TEM). Rats administered with D-gal and AlCl3 exhibited cognitive deficits paralleled with marked increase in AChE activities and MDA level in cerebral cortex and hippocampus and decreased SOD activities. Further, a glaring ultrastructural morphological alterations in cerebral cortex were observed. Conversely, CA administration not only reversed the cognitive impairment but also decreased AChE activities and MDA level in hippocampus and cerebral cortex of D-gal and AlCl3 induced rats, it also increased the activities of SOD and prevented morphological alteration in cerebral cortex. Irrespective of dose administered, the protective effect of CA is comparable to donepezil. In conclusion, this study suggest that CA attenuated cognitive deficits in rats probably by restoring cholinergic function, attenuating oxidative stress and by preventing morphological aberration.

Keywords: Centella asiatica; cognitive deficit; Alzheimer’s disease; morphological aberration; oxidative stress.
Requires Audio or Video system for Presentation?: No