MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

Molecular detection of Ascaris species from human and swine in indigenous communities in Sarawak, Malaysia

Type
E-Poster Presentation Competition for Student
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Nur Amirah Hassan1
Presenting Author
Nur Amirah Hassan1
Co-Author
Yvonne Ai Lian Lim1
Romano Ngui1
Autchariya Thodsapol2
Yamuna Rajoo1

Authors' Institution

Department / Institution / Country
Department of Parasitology / University of Malaya / Malaysia1
Department of Microbiology / Prince of Songkla University / Thailand (ไทย)2
Content
Abstract Content

Ascaris (roundworm) are soil-transmitted helminths (STHs) that causing ascariasis in human. The infections are widely distributed throughout the tropical and subtropical countries including Malaysia, particularly among underprivileged communities. Most of the study established the distribution of the roundworm in both human and animal hosts. However, the molecular epidemiology of Ascaris in both hosts still is limited in Malaysia. This study was conducted to determine the prevalence of Ascaris spp. from human and pig among indigenous communities in Sarawak. A cross-sectional study was conducted among longhouses communities. A total of 341 human and 25 swine stool samples were collected and examined for the presence of Ascaris using microscopy technique. Microscopy positive sample was subjected to polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP) and DNA sequencing. The overall prevalence of Ascaris was reported as 19.9% in human and 64% in the pig as determined microscopy examination. Our initial molecular analysis showed that 63.2% were identified as Ascaris lumbricoides in the human stool samples. In swine stool sample, 75% were detected as Ascaris suum. Sequence analysis is still ongoing to determine the potential of cross-transmission of both species in human and swine samples. Essentially, this finding highlighted that ascariasis is prevalent among the rural indigenous community in Sarawak as well as in animal hosts. Improvement of socioeconomic status, periodic mass deworming and health education program should be included in the control and prevention of public health strategies. 

Keywords: Ascaris lumbricoides; Ascaris suum; PCR-RFLP; Indigenous communities; Sarawak
Requires Audio or Video system for Presentation?: No