MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur



Monitoring of Wolbachia-infected Ae. aegypti and wild field Ae. aegypti against diagnostic dosages of permethrin 0.25% and fenitrothion 1.0%.

Oral Presentation
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Vector Biology & Control


Main Author
Siti Nor Syazwani Amran1
Presenting Author
Siti Nor Syazwani Amran1
Nur Ruqqayah Mohd Mazni1
Noor Afizah Ahmad1
Nazni Wasi Ahmad1
Han Lim Lee1

Authors' Institution

Department / Institution / Country
Medical Entomology Unit, WHO Collabrating Centre for Ecology, Toxonomy & Control Vectors of Malaria, / Institute for Medical Research / Malaysia1
Abstract Content

Wolbachia-infected Ae. aegypti are unable to support development of dengue virus and these mosquitoes were released in several dengue hotspots in Selangor to replace the wild Ae. aegypti in order to interrupt dengue transmission.  As a result of Wolbachia release, it is crucial to monitor the insecticide susceptibility status of Wolbachia carrying and wild Ae. aegypti mosquito population where the invasion takes place. Comparable susceptibility status of both strains would ensure that the Wolbachia-infected Ae. aegypti populations are able to survive if chemical insecticide spraying was conducted in the Wolbachia release area. The susceptibility status of both adult Wolbachia-infected Ae. aegypti and wild  field Ae. aegypti were conducted against the diagnostic dosage of permethrin 0.25% and fenitrothion 1.0% in accordance to the WHO bioassay procedures. Eggs were collected after bloodfeeding and hatched on the same day and reared to adults. Adult females aged 3-5 days old  were exposed to the insecticide impregnated paper and the mortality counts were made 24 hours post-exposure.The results showed that  the mean percentage mortality against fenitrothion was much higher compared to permethrin in both strains, indicating presence of resistance to permethrin. This could be due  to the extensive and prolonged usage of permethrin for dengue control during outbreaks. Thus, the results obtained from Wolbachia screening it is concluded that Wolbachia-infected Ae. aegypti are able to survive within field population.

Keywords: Wolbachia, Insecticide, Permethrin, Fenitrothion, Mosquitoes
Requires Audio or Video system for Presentation?: Yes