MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur




E-Poster Presentation Competition for Student
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Emerging Zoonoses


Main Author
Norliza Bahtiar Affendy1
Presenting Author
Norliza Bahtiar Affendy1
Mohd Nasir Mohd Desa1
Fairuz Amran2
Siti Norbaya Masri1

Authors' Institution

Department / Institution / Country
Medical Microbiology and Parasitology / Universiti Putra Malaysia / Malaysia1
Bacteriology Unit / Institute of Medical Research / Malaysia2
Abstract Content

Introduction: Leptospirosis is an emerging zoonotic disease in tropical regions including Malaysia. Rats are known as the most important reservoir and source of transmission of the disease. To date, the status of leptospirosis in Malaysia related to the reservoir and transmission is still underestimated.

Objectives: To determine the prevalance of leptospirosis among small mammals via microscopic agglutination test (MAT) and isolation of the organisms through culture method. The study also aims to determine the species of small mammals carrying leptospires.

Methodology: A total of 89 rodents were collected from two wet markets located in Selangor. Serum samples were tested for leptospiral antibodies using MAT against a panel battery of 20 live serovars. Any sample having titer ≥100 to any tested serovar are considered as positive result. Supernatant of crushed kidneys were cultured in liquid Ellinghhausen McCullough Johnson Harris (EMJH) medium, incubated at room temperature for 3 months and examined under the dark-field microscope. Positive growth of the leptospiral isolates were then confirmed with the present of flaB gene through polymerase chain reaction (PCR).

Discussion: The overall prevalence of leptospirosis was 28.1% (n=25). The seropositivity by MAT was 18.0% (n=16) whereas by culture was 16.9% (n=15).  The serum samples reacted to 2 serovars with Bataviae as the predominant serovar identified. This investigation highlight the robust present of pathogenic leptospires among rats compared to shrew which may present the risk of infection, especially among high risk individual in the studied sites. 

Conclusion: This preliminary result showed a relatively high seroprevalence of leptospirosis among small mammals in Selangor, Malaysia indicating the need to strengthen the preventive measures and policies of this rat urine disease especially in a high-risk area like wet markets.


Keywords: prevalance; pathogenic leptopsira; MAT; isolate; wet markets
Requires Audio or Video system for Presentation?: No