MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

EXPRESSION PROFILE OF ASCARIS LUMBRICOIDES PUTATIVE VIRULENCE-RELATED GENES WHEN INFECTING A HUMAN HOST

Type
Oral Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Norashikin Mohd. Shaharuddin1
Presenting Author
Norashikin Mohd. Shaharuddin1
Co-Author
Romano Ngui1
Yvonne Ai Lian Lim1
Sheila Nathan2

Authors' Institution

Department / Institution / Country
Parasitology / University Of Malaya / Malaysia1
School Of Bioscience And Biotechnology / Universiti Kebangsaan Malaysia / Malaysia2
Content
Abstract Content
Ascaris lumbricoides is one of the principal causative agents of soil-transmitted helminth (STH) infections worldwide. Host susceptibility and genetics play an important role in the distribution of the parasite. The helminths are capable to evade and manipulate the host immune system in a variety of ways through the release of a spectrum of immunomodulatory factors which underpins their long-term survival in the host. However, little is known on the evasion strategies of A. lumbricoides when infecting a human host. In this study, quantitative polymerase chain reaction (qPCR) was performed to evaluate the expression profile of A. lumbricoides putative virulence-related genes from the infected children and adults. A total of 12 putative genes were selected pertaining to virulence-related genes reported from previous studies. The study was initiated by collecting adult worms expelled from adult and children following anthelminthic treatment. The RNA integrity number (RIN) of the extracted A. lumbricoides ranged from 6.5 to 7.7. The primers designed for A. lumbricoides showed specific amplicons. The expression profile of putative virulence-related genes in A. lumbricoides from infected adults was compared to A. lumbricoides from infected children (control) demonstrated that VENOM was highly up-regulated (166-fold), p<0.05 whilst the expression of CADHERIN and PEBP was elevated significantly at 13-fold and 5-fold, respectively. The transcription of ABA-1 (4-fold), CATH-L (3-fold) and INTEGRIN (2-fold) was suppressed significantly in A. lumbricoides infected adults. In addition, the relative expression of the 12 genes against housekeeping genes showed that CYSTATIN was abundantly expressed in A. lumbricoides from infected adults and infected children. Based on the previous studies conducted on a variety of parasitic helminths, it is suggested that these proteins may play significant immunomodulatory roles during infection. Moreover, the elucidation of the virulence-related gene expression could be fundamental for the development of novel therapeutic interventions in the future.rir
Keywords: Ascaris lumbricoides; expression; virulence genes; immunomodulatory
Requires Audio or Video system for Presentation?: No