MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

THE IN VITRO ANTIMALARIAL AND TOXICOLOGICAL ACTIVITIES OF QUERCUS INFECTORIA OLIVIER (MANJAKANI) CRUDE EXTRACTS

Type
Oral Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Malaria

Authors

Main Author
Nik Nor Imam Nik Mat Zin1
Presenting Author
Nik Nor Imam Nik Mat Zin1
Co-Author
Mira Nabila Mohamad1
Nurul I'zaaz Abdul Moin2
Azamuddin Alias2
Abdul Wafi Sazeli3
Yusmazura Zakaria1
Nurhidanatasha Abu Bakar1
Keusar Roslan1

Authors' Institution

Department / Institution / Country
School of Science / Universiti Sains Malaysia / Malaysia1
Department of Biomedical Sciences / Universiti Islam Antarabangsa Malaysia / Malaysia2
Institute of Health Sciences / Universiti Brunei Darussalam / Brunei3
Content
Abstract Content
 

Abstract Content:

There is an urgent need for the discovery of new antimalarial drugs as most currently available drugs for malaria treatment have been threatened the development of single and multi-drug-resistant parasite populations. Herein, the in vitro antimalarial and toxicological activities of the medicinal plant, Quercus infectoria Olivier (manjakani) gall crude extracts were investigated. Four crude extracts of different polarity of solvents (acetone, ethanol, methanol and aqueous) were prepared and screened for the antimalarial activity using a malarial SYBR Green 1 fluorescence-based (MSF) assay against asexual blood stages of a chloroquine-sensitive strain (3D7) of Plasmodium falciparum. The crude extracts were evaluated for their toxicity using brine shrimp lethality test (BSLT), haemolytic assay, and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assay against mouse embryo fibroblast cell line (NIH/3T3) and Vero epithelial cell (CCL-81), which the selectivity index (SI) was determined. Acetone and methanol extracts show a promising antimalarial activity with a half-maximal inhibitory concentration (IC50) of 5.85 ± 0.22 μg/mL and 10.31 ± 0.27 μg/mL, respectively. All the crude extracts were nontoxic to brine shrimps with a half-median lethality concentration (LC50) of >1000 ppm. No occurrence of haemolysis was observed on normal erythrocytes (<10%) treated with all the crude extracts. However, all the crude extracts show a moderate cytotoxic to both NIH/3T3 and CCL-81 cells with a half-maximal cytotoxicity concentration (CC50) of <90 µg/mL. Taken together their SI values less than 10, this infers that all the crude extracts have selectively suppressed the development of different cell types. Overall, the results demonstrate that the crude acetone and methanol extracts of Q. infectoria galls have the potential ethnobotanical antimalarial properties. The isolation of bioactive constituents from these extracts are encouraged to investigate the molecular mechanism underlying the antimalarial effect of the Q. infectoria galls.
Keywords:
Requires Audio or Video system for Presentation?: Yes