MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

ASSESSMENT OF VIRULENCE FACTORS OF POLYKETIDE SYNTHASE (PKS)-POSITIVE KLEBSIELLA PNEUMONIAE ISOLATED FROM COLORECTAL CANCER PATIENTS

Type
E-Poster Presentation Competition for Student
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Christina Kaur1
Presenting Author
Christina Kaur1
Co-Author
Jamuna Vadivelu1
Chandramathi Samudi1

Authors' Institution

Department / Institution / Country
Department of Medical Microbiology / University of Malaya / Malaysia1
Content
Abstract Content

Colorectal cancer (CRC) is the second most common cancer affecting Malaysians. Infectious agents such as bacteria are responsible for approximately 20% of all cancers worldwide. Klebsiella pneumoniae (K. pneumoniae), is one of the most common pathogen isolated from patients with lower gastrointestinal tract (GIT) diseases. The PKS gene is responsible for the production of the genotoxin colibactin which is able to cause double strand DNA damage in host cells. Numerous studies have shown the association of K. pneumoniae with CRC as a subsequence of pyogenic liver abscess (PLA) which is a precursor condition for the development of CRC. However, studies directly linking K. pneumoniae in the development and progression of CRC is still scanty. This study intends to investigate the association of PKS-positive K. pneumoniae virulence factors with CRC progression. K. pneumoniae isolates obtained from CRC patients and healthy individuals were subjected to PKS & virulence gene detection, biofilm assay, string test and antibiotic susceptibility testing. 25% of the isolates carried the PKS gene and majority of these isolates were moderate to strong biofilm producers. 37.5% of the isolates had a positive string test (hypermucoviscosity). Biofilm producing hypermucoviscosity PKS-positive isolates which had increased virulence were isolated from initial stage CRC patients, whereas less virulent strains were obtained from more advanced CRC patients. These findings suggest that K. pneumoniae virulence factors do play a role in attachment and colonization of the lower GIT to inflict damage to the colonic mucosa in initial stages of CRC as a driver bacterium compared to more advanced stages of CRC where less virulent strains play a role as passenger bacterium. PKS-positive K. pneumoniae and its associated virulence factors could possibly play a role in enhancing CRC progression to more advanced forms. However, further investigations are warranted to substantiate these findings and will be discussed further.

Keywords: Colorectal cancer; Klebsiella pneumoniae; Lower Gastrointestinal Tract Diseases; Polyketide Synthase;
Requires Audio or Video system for Presentation?: Yes