MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

THE ROLE AND INFLUENCE OF BACTERIA ON THE GROWTH OF BLASTOCYSTIS SP. SUBTYPE 3

Type
E-Poster Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Chai Yong Chia1 3
Presenting Author
Chandramathi Samudi1
Co-Author
Chandramathi Samudi1
Suresh Kumar2
Christina Parvinder Kaur1

Authors' Institution

Department / Institution / Country
Department of Medical Microbiology / University of Malaya / Malaysia1
Department of Parasitology / University of Malaya / Malaysia2
Department of Biomedical Science,Faculty of Medicine / University of Malaya / Malaysia3
Content
Abstract Content

Blastocystis sp. is known as one of the most common intestinal protozoa found in any population. There are more than 17 specific small-subunit ribosomal RNA subtypes, of which nine are found in humans. Blastocystis sp. Subtype 3 has been often associated with various gastrointestinal diseases namely Irritable Bowel Syndrome, Crohn’s disease and ulcerative colitis. Symptoms caused by Blastocystis infection include abdominal cramps, diarrhea, flatulence and anorexia. However, the pathogenicity of this parasite is usually queried as the parasite, including Subtype 3, can be present in asymptomatic individuals as well. Morphological and metagenomics studies on this organism have revealed the presence of other accompanying bacteria in its in vitro culture. Furthermore, axenization of this parasite is not possible especially for Subtype 3 as the parasite will not survive without the presence of accompanying bacteria. These observations lead to the speculation that the parasite’s pathogenicity may be contributed by the types of accompanying bacteria. Therefore, the present study aimed to identify and elucidate the role of accompanying bacteria towards the growth of Blastocystis sp. Subtype 3 in vitro. This objective was achieved by using Subtype 3 parasite cultures that were obtained from symptomatic and asymptomatic individuals. The role of accompanying bacteria was accessed by studying the parasite’s growth profile and morphological changes upon exposure to different types of bacteria by introducing the parasite cultures to various types of antibiotics targeting the elimination of gram positive and/or gram negative bacteria. The parasite was co-cultured with various colony forming unit (CFU) of selected bacteria in order to see if the growth of parasites is enhanced. The experimental results of this study will be discussed further.

Keywords: Blastocystis sp., symptomatic, asymptomatic, accompanying bacteria
Requires Audio or Video system for Presentation?: No