MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

EMERGENCE OF PARABURKHOLDERIA FUNGORUM AS AN OPPORTUNISTIC BACTERIAL PATHOGEN

Type
E-Poster Presentation Competition for Student
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Shih Keng Loong1
Presenting Author
Shih Keng Loong1
Co-Author
Yih Harng Soh2
Kim-Kee Tan1
Sazaly AbuBakar1

Authors' Institution

Department / Institution / Country
Tropical Infectious Diseases Research & Education Centre / Universiti Malaya / Malaysia1
Department of Pathology / Hospital Melaka / Malaysia2
Content
Abstract Content

Paraburkholderia fungorum is an opportunistic bacteria infrequently associated with human infections. P. fungorum is ubiquitously found in the environment with previously reported isolation from animal and human clinical specimens. Although this outdoor, environment-associated bacteria species is generally innocuous to humans, we recovered an isolate from a patient’s knee wound specimen. Clinical investigation revealed that the patient suffered from a long term sports-related injury to the inflicted knee and sought treatment when the knee was severely injured from a fall, two years later. Synovial fluid was turbid, hence infection of the synovial tissue was suspected and culture of the tissue biopsy grew P. fungorum, sensitive to commonly used antibiotics. Characterization using multilocus sequence typing found novel alleles corresponding to a novel sequence type 868. Complete genome sequencing of P. fungorum revealed the presence of antimicrobial resistance genes against the beta lactams (beta lactamase Class A, Class C and penicillin binding proteins), alongside genes encoding for extracellular antibacterial colicin and other bacteriocins. Amino acid sequence analyses revealed that P. fungorum contained the following conserved sequence indels (CSI); transposase A-like protein, group 1 glycosyl transferase and undecaprenyl-phosphate glucose phosphotransferase. These are molecular signatures unique to the genus Paraburkholderia. We suspect that the patient possible acquired P. fungorum during his initial injury, very likely from the environment. Our observations suggest the ability of P. fungorum to act as a slowly replicating opportunistic pathogen associated with tissue injuries. These findings and the genome sequence data could potentially be helpful to other investigators for comparative genomics studies of other P. fungorum strains to provide insights into the possible mechanisms of pathogenesis.

Keywords: Environment, infectious disease, soil, tropical
Requires Audio or Video system for Presentation?: Yes