MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

THE USEFULNESS OF SALIVA AND URINE SAMPLES FOR MALARIA DIAGNOSIS

Type
E-Poster Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Malaria

Authors

Main Author
Hwa Chia Chai1
Presenting Author
Hwa Chia Chai1
Co-Author
Kek Heng Chua1
Narong Jaturas2
Veeranoot Nissapatorn2

Authors' Institution

Department / Institution / Country
Biomedical Science / University of Malaya / Malaysia1
Parasitology / University of Malaya / Malaysia2
Content
Abstract Content
Malaria remains one of the most important communicable diseases and blood is still the preferred specimen for molecular malaria diagnosis. Blood collection can be a hazard for some patients. Hence, this study intended to investigate the efficiency of Plasmodium falciparum (PF) and P. vivax (PV) DNA detection from samples collected in less invasive means, which were the saliva and urine, by using a sensitive molecular method, which is the real-time PCR. Matched blood, saliva and urine samples were collected from 71 microscopically positive patients who attended a selected malaria clinic in southern Thailand. Real-time PCR targeting the small subunit ribosomal RNA gene of human malaria were performed. Of the 34 (47.9%) microscopically PF detected blood samples, only 30 (88.2%) were detected to contain PF DNA, 2 (5.9%) contained mixed DNA from PF and PV and the remaining 2 (5.9%) were negatively detected. While for PV, only 30 (81.2%) out of 37 (52.1%) microscopically detected blood samples were positive for PV DNA, 3 (8.1%) were positive for mixed DNA from PF and PV and none was detected in 4 (10.8%) samples. However, real-time PCR could only detect 8 (26.7%) saliva and 3 (10.0%) urine samples with PF DNA, as well as 2 (6.7%) saliva and 1 (3.3%) urine samples with PV DNA. This study showed the attempt of PF and PV DNA in saliva and urine samples real-time PCR. Nevertheless, the results showed the need for improvements in plasmodial DNA detection in non-invasive specimens, which include the DNA extraction methods, sample preservation, primer/probe set and fraction/volume of sample used, before they can be used as alternative specimens for malaria diagnosis.
Keywords: Malaria diagnosis, saliva, urine, Plasmodium falciparum, Plasmodium vivax, real-time PCR
Requires Audio or Video system for Presentation?: No