MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur




Oral Presentation
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Medical Microbiology & Parasitology


Main Author
M.N Wana1 5
Presenting Author
M.N Wana1 5
Mohamad A. M.M2
Malaika W4
Ngah Z. U1
Sharif A.A1 6
Ashraf A. Alapid1 3
Tijjani M1 7
Shariza N3
Rusliza B2
Roslaini AM1

Authors' Institution

Department / Institution / Country
Department of Medical Microbiology and Parasitology / Faculty of Medicine and Health Sciences / Malaysia1
Human Anatomy / Universiti Putra Malaysia / Malaysia2
Department of Biomedical Sciences / Faculty of Medicine and Health Sciences / Malaysia3
Department of Veterinary Clinical Studies / Faculty of Veterinary Medicine, Universiti Putra Malaysia / Malaysia4
Department of Biological Sciences / Faculty of Science, Abubakar Tafawa, Balewa, University Bauchi, Nigeria / Nigeria5
Department of Medical Microbiology and Parasitology / Faculty of Clinical Sciences, Bayero University, Kano, Nigeria / Nigeria6
Dept. of Biological Sciences / Yobe State University, Damaturu, Nigeria / Nigeria7
Abstract Content

Toxoplasma gondii has the predilection to the brains of its intermediate host leading to latent toxoplasmosis with variable outcomes. Three different strains have been identified worldwide with various degree of susceptibility and clinical outcomes. This study aimed to evaluate the effects of Malaysian isolates of T.gondii oocysts in rats. Rats were infected with either Type l, Type ll & Type lll strains of T.gondii while control groups received either MK-801 or PBS for 11 weeks. The rats were tested for locomotor activities, anxiety, spatial learning and memory through open field test, elevated plus maze (EPM) and morris water maze (MWM) test respectively. Further, the odour discriminatory abilities of the rats were also evaluated by exposing them to urines of cats, dogs, rats and water respectively. The test for locomotor activities and odour discrimination were conducted in the 9th week post infection (pi), while the tests for anxiety and cognitive abilities were conducted in the 10th and 11th weeks pi. There were no differences observed in the locomotor activities in all the group of rats, however, attraction to cat’s urine odour were observed among the infected groups when compared to the control groups. Additionally, the rats exposed to MK-801 and Type l, Type ll & Type lll strains of T.gondii spent more time in the open arm of EPM indicating less anxiety when compared to the PBS group. Finally, all groups of rats performed equally in the training component of the MWM test, nevertheless only the PBS treated group performed well in the probe trial component of the MWM test as they spent more time in the target quadrant. In conclusion, the Malaysian isolates of T.gondii induced fear, anxiety and cognitive deficits in rats. Studies are underway to elucidate the probable mechanisms of molecular pathways of T.gondii in rat infection.  

Keywords: Toxoplasma gondii; albino wistar rat; anxiety; spatial learning and memory; odour discriminatory test.
Requires Audio or Video system for Presentation?: Yes