MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur




Oral Presentation
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Veterinary Parasitology


Main Author
Nor Azlina Abdul Aziz1
Presenting Author
Quincie Sipin1
Quincie Sipin1
Malaika Watanabe2
Puteri Azaziah Megat Abdul Rani2
Farina Mustaffa Kamal1
Nur Mahiza Md Isa1
Reuben Sunil Kumar Sharma3

Authors' Institution

Department / Institution / Country
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine / Universiti Putra Malaysia / Malaysia1
Department of Medicine & Companion Animal Surgery, Faculty of Veterinary Medicine, / Universiti Putra Malaysia / Malaysia2
Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine / Universiti Putra Malaysia / Malaysia3
Abstract Content
The present study was designed to investigate some of the most important tick-borne haemopathogens of dogs in Peninsular Malaysia. Blood samples were collected from 220 dogs, randomly selected from 10 animal shelters located in Peninsular Malaysia. DNA were extracted and amplified by conventional PCR using species-specific primers that amplify the 400bp fragment of the 16SrRNA gene for Ehrlichia canis and the 500bp fragment of the 16SrRNA gene for Anaplasma platys, and a genus-specific primer that amplifies the 450bp fragment of the 18SrRNA gene for Babesia spp. Statistical analyses were performed using SPSS (23), where Chi-square test was used for the relation between categorical outcomes, i.e. absence or presence of specific infection, sex, and age, and p≤0.05 was considered to indicate a statistically significant difference. The results showed that 35% (n=77) of the collected samples were infected with at least one of the three hemopathogens, of which Ehrlichia canis predominated with a prevalence of 20% (n=43; CI=0.1465-0.2554), followed by Babesia spp., and Anaplasma platys with prevalence rates of 15% (n=32; CI=0.1031-0.2007),  and 12% (n=26; CI=0.0801-0.1701), respectively. Male dogs showed a significantly  higher Anaplasma platys infection rate compared to their female counterparts (P≤0.05)  while no significant different observed between sex group for Ehrlichia canis and Babesia spp. There was no significant difference among age group for all the hemopathogens. Single infection of haemopathogens is common (25%; n=54). Presence of co-infection with two haemopathogens (10%; n=22) was also established of which Anaplasma platys and Ehrlichia canis were most common to co-infect (6%; n=12). The study highlighted the current molecular prevalence of tick-borne hemopathogens from the blood sample taken in stray dogs with E. canis as the main contributor as well as the presence of co-infection haemopathogens in Peninsular Malaysia.
Keywords: Stray dogs, Peninsular Malaysia, Tick-borne hemopathogens, PCR
Requires Audio or Video system for Presentation?: No