MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

FIRST REPORT OF WHOLE GENOME SEQUENCING OF RIFAMPICIN RESISTANT MYCOBACTERIUM TUBERCULOSIS IN SABAH, MALAYSIA

Type
Oral Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Jaeyres Jani1
Presenting Author
Jaeyres Jani1
Co-Author
Zainal Arifin Mustapha2
Norfazirah binti Jamal4
Cheronie Shely Stanis4
Chin Kai Ling3
Richard Avoi5
Oo Tha Naing5
Twee Hee Rick Ong6
Valentine Gantul7
Kamruddin Ahmed1 4

Authors' Institution

Department / Institution / Country
Borneo Medical and Health Research Centre / Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah / Malaysia1
Department of Medical Education / Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah / Malaysia2
Department of Biomedical Sciences and Therapeutic / Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah / Malaysia3
Department of Pathobiology and Medical Diagnostics / Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah / Malaysia4
Department of Community and Family Medicine / Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah / Malaysia5
Saw Swee Hock School of Public Health, / National University of Singapore / Singapore6
Tuberculosis and Leprosy Control Unit / Sabah State Health Department,Kota Kinabalu / Malaysia7
Content
Abstract Content

According to WHO report of Oct 2017, tuberculosis (TB) is one of the top 10 causes of death worldwide. In 2016, 10.4 million people became infected with TB, and 1.7 million died from the disease (including 0.4 million among people with HIV). In Malaysia,25,747 cases were reported in 2016 which is 6.3% higher than in 2015. Among all the states in Malaysia, Sabah contributed 19.5% (5106) of the total cases of TB in 2017. The most challenging part of TB control is drug resistance TB. In 2016, an estimated 490,000 people worldwide developed multi-drug resistance (MDR)-TB, and an additional 110,000 people with rifampicin-resistant TB were also newly eligible for MDR-TB treatment. Malaysia a not a high burden MDR-TB country. In 2017, 370 cases of MDR-TB have been reported from Malaysia and 8 cases were contributed from Sabah. Studies on the genetic analysis of MDR-TB is scarce not only in Sabah but in Malaysia as a whole.

Studies have continuously highlighted the presence of novel mutations in genes with previously reported mutations (rpoA, rpoB, rpoC), which suggests the possibility of new information available within the locally acquired isolates. Furthermore, bioinformatics is one of the approaches which can predict the mutation and functional effects of these mutations via computational modeling. Whole genome sequencing (WGS) of the TB isolates was carried out via Next-Generation Sequencing (NGS).

Our M. tuberculosis isolate was identified as rifampicin resistance using GeneXpert. After WGS, bioinformatics analysis was performed. The de novo assembly of genome generated 127 contigs with N50 of 156, 381. The GC contents is 65.6% and consists of 4,088 predicted genes. The mutation site for rifampicin resistance was detected in S450L in rpoBgene. Our strain belongs to Europe America Africa (Lineage 4) based on phylogenetic analysis using neighbor-joining method.

Keywords:
Requires Audio or Video system for Presentation?: No