MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

AEDES MOSQUITOES RESISTANCE STATUS IN KELANTAN AFTER THE NATURAL FLOOD DISASTER

Type
Oral Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Vector Biology & Control

Authors

Main Author
Wan Fatma Zuharah Wan Musthapa1
Presenting Author
Wan Fatma Zuharah Wan Musthapa1
Co-Author
Ahbi Rami Rattanam1
Izhan Shahrin Jaafar2
Zary Shariman Yahaya1
Intan Haslina Ishak1

Authors' Institution

Department / Institution / Country
School of Biological Sciences / Universiti Sains Malaysia / Malaysia1
Vector Control Research Unit Kota Bharu District / Ministry of Health / Malaysia2
Content
Abstract Content

In December 2014 the people of the east coast Malaysia, especially in Kelantan faced a sudden hit of flood disaster inflicted enormous human and physical damaged which information related to mosquitoes emergence and natural disaster remains sparse. Therefore, our team conducted the entomological surveillance of Aedes in a monthly basis for a year between 2015 and 2016 on selected localities: Pasir Pekan, Tumpat (flooded area) and compared with Bandar Baru Kubang Kerian (unflooded area). Overall, flooded area showed higher OI (85.03%) compared to unflooded area (62.82%), indicating higher mosquito abundance in the former (P<0.05). There were no correlations observed between mosquito population, rainfall, temperature, humidity, fogging frequency and number of dengue cases in both flooded and unflooded areas (P>0.05). We found that higher mosquito abundance does not necessarily oblige to cause elevated dengue cases. Mosquitoes collected from both areas were subjected to bio-assay following WHO protocol against deltamethrin (0.05%) and pirimiphos-methyl (0.25%) to evaluate their susceptibility status. The mosquitoes from both locations were found to be susceptible to pirimiphos-methyl (mortality >95%). High level of resistance was detected in Ae. aegypti from both areas, with mortality of 74.0 ± 1.29 and 17.0 ± 0.96, respectively, when tested with deltamethrin. Investigation of kdr mutation showed F1534C substitution in VGSC gene of Ae. aegypti from both locations, with the frequency of more than 75%, while none detected in Ae. albopictus. No other kdr mutation was detected in domain II (S989, I1011, L1014, and V1016) for both species. Both flooded and unflooded areas showed pyrethroid resistance phenotypes and genotypes, with higher frequency in the unflooded area. The overall results provide baseline data for systematic planning and insecticide selection before the commencement of controlling operational activities, especially after a natural disaster.

Keywords: Aedes; Flood disaster; kdr mutation; Pyrethroid; Resistance
Requires Audio or Video system for Presentation?: Yes