MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

HIGH SENSITIVITY OF DETECTION OF HUMAN INFECTIONS WITH PLASMODIUM KNOWLESI BY A RAPID DIAGNOSTIC TEST (RDT)

Type
Oral Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Malaria

Authors

Main Author
Hanisah Hossain1
Presenting Author
Hanisah Hossain1
Co-Author
Khamisah Abdul Kadir1
Dayang Shuaisah Awang Mohamad1
Nawal Rosli1
Balbir Singh1

Authors' Institution

Department / Institution / Country
Faculty of Medicine and Health Sciences, Malaria Research Centre / Universiti Malaysia Sarawak / Malaysia1
Content
Abstract Content
In Malaysia, Plasmodium knowlesi accounted for 88% of the 4,114 reported cases of malaria in 2017 and is a major public health problem. The blood stages of P. knowlesi and P. malariae are morphologically similar under the microscope. Although molecular detection assays are essential for correct diagnosis, they are relatively expensive, are not rapid and require electricity, making them unsuitable for rural health settings. Rapid diagnostic tests (RDTs) provide a viable alternative and the aim of the study was to evaluate a new RDT, the Parascreen Pan/Pf RDT, for the detection of P. knowlesi. One hundred and thirty-nine frozen blood samples were randomly selected from 534 PCR-confirmed single P. knowlesi-infected samples to evaluate the RDT. The P. knowlesi-infected samples were identified as pan-malaria infections by the RDT and no cross-reactivity was observed against the P. falciparum test reagent. The overall sensitivity of the RDT towards P. knowlesi was 81.3% (113/139; 95% CI = 81.2-81.4%). The sensitivity of detection for samples with low parasitemia (100-1,000 parasites/µl) was 57.4% (27/47; 95% CI = 57.3-57.6%), for those with moderate parasitemia (1,001-5,000 parasites/µl), it was 95.7% (44/46; 95% CI = 95.6-95.7%) and for those with high parasitemia (>5,000 parasites/µl), it was 91.3% (42/46; 95% CI = 91.2-91.4%). The overall sensitivity of detection of P. knowlesi (81.3%) with the Parascreen Pan/Pf RDT was higher than that reported for the following RDTs that have been evaluated so far: First Response (73.6%; 95/129), CareStart Malaria Combo (42.1%; 75/178); OptiMAL-IT (39.1%; 90/230); Paramax-3 (31.7%; 13/41); BinaxNOW Malaria (24.4%; 10/41); ParaHIT (22.9%; 22/96). Further work needs to be undertaken to assess the performance of Parascreen Pan/Pf RDT in the field with fresh blood samples and more sensitive RDTs are required for the diagnosis of P. knowlesi malaria in rural health settings.
Keywords: Plasmodium knowlesi; Malaria; Rapid diagnostic tests, Diagnosis
Requires Audio or Video system for Presentation?: Yes