MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur



Identification Of E. Bieneusi Circulating Proteins From HIV/AIDS Patients With Disseminated Microsporidiosis Via Shotgun Proteomic Approach

E-Poster Presentation
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Medical Microbiology & Parasitology


Main Author
Emelia Osman1
Presenting Author
Emelia Osman1
Nurul Shazalina Zainudin1
Rahmah Noordin2
Nurul Hasanah Othman2
Asmahani Azira Abdu Sani3
Wan Mohd Aizat Wan Kamaruddin4
Petrick Ramesh K. Periyasamy5

Authors' Institution

Department / Institution / Country
Parasitology & Medical Entomology / Universiti Kebangsaan Malaysia / Malaysia1
Institute for Research in Molecular Medicine / Universiti Sains Malaysia / Malaysia2
Malaysia Genome Institute / Malaysia Genome Institute / Malaysia3
Institute of Systems Biology / Universiti Kebangsaan Malaysia / Malaysia4
Department of Medicine / Universiti Kebangsaan Malaysia / Malaysia5
Abstract Content



Microsporidia are intracellular parasites that are highly-derived relatives of fungi. Medical relevance of microsporidia was appreciated when they were found to be responsible for lethal diarrhoea in AIDS patients, and death in transplant and immunocompromised patients. Enterocytozoon bieneusi and Encephalitozoon spp. are the two important microsporidia species with E. bieneusi reported to be the most common microsporidial infection in man. To date, there are increasing reports on disseminated microsporidiodis worldwide. Diagnosis of microsporidia infection has traditionally been performed via microscopy based on examination of faeces and other body fluids. However, in disseminated cases, the infection is overlooked since the organisms are small (1-4 micron), requiring expertise by microscopists in diagnostics laboratories. This study aimed to detect and identify E. bieneusi circulating proteins from sera of HIV/AIDS patients with disseminated microsporidiosis. A total of 100 blood samples were collected from HIV/AIDS patients in Sungai Buloh Hospital, Selangor. PCR amplification was performed to detect circulating E. bieneusi DNA in all blood specimens of HIV/AIDS patients. Out of 100 samples, 7 were confirmed positive with E. bieneusi DNA. A gel-free shotgun proteomics analysis was then used to detect E. bieneusi circulating protein(s) in serum samples following the removal of albumin and IgG serum proteins. Two unique proteins were identified consistently in all positive E. bieneusi serum samples. This study proves the presence of circulating E. bieneusi proteins in sera of HIV/AIDS patients with disseminated microsporidiosis. To the best of our knowledge, this is the first study to report on identification of circulating E. bieneusi proteins via serum proteomic approach. Thus, the data is useful for further studies on development of diagnostic and therapeutic target for disseminated microsporidiosis

Keywords: E. bieneusi, HIV, serum, shotgun proteomic, circulating DNA, microsporidiosis
Requires Audio or Video system for Presentation?: No