MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

DEVELOPMENT OF AN ANTIGEN DETECTION ELISA FOR BANCROFTIAN FILARIASIS USING BMSXP-SPECIFIC RECOMBINANT MONOCLONAL ANTIBODY

Type
Oral Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Neglected Tropical Diseases

Authors

Main Author
Rahmah Noordin1
Presenting Author
Rahmah Noordin1
Co-Author
Anizah Rahumatullah 1
Theam Soon Lim1
Muhammad Hafiznur Yunus1

Authors' Institution

Department / Institution / Country
Institute for Research in Molecular Medicine (INFORMM) / Universiti Sains Malaysia / Malaysia1
Content
Abstract Content
Lymphatic filariasis is a mosquito-borne parasitic disease responsible for morbidity and disability that affects 1.2 billion people worldwide, mainly the poor communities. Currently, filarial antigen testing is the method of choice for detection of bancroftian filariasis, and to date, there are two commonly used tests. In the present study, a recently reported recombinant monoclonal antibody (5B) specific to BmSXP filarial antigen was used in developing an ELISA for detection of circulating filarial antigen in sera of patients with bancroftian filariasis. The performance of the ELISA was evaluated using 75 serum samples. The ELISA was positive with all sera from microfilaremic bancroftian filariasis patients (n=30). It also showed 100% diagnostic specificity when tested with sera from 30 healthy individuals and 15 patients with other parasitic diseases. The developed assay using the novel 5B recombinant monoclonal antibody could potentially be a promising alternative antigen detection test for bancroftian filariasis.
Keywords: Lymphatic filariasis; Wuchereria bancrofti; Recombinant monoclonal antibody; Antigen detection ELISA2
Requires Audio or Video system for Presentation?: Yes