MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

APPLICATION OF THE ESTABLISHED PROTOCOL FOR THE DETECTION OF BLASTOCYSTIS SP. IN WATER SAMPLES

Type
Oral Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Ii Li Lee1
Presenting Author
Ii Li Lee1
Co-Author
Tian Chye Tan2
Suresh Kumar Govind2

Authors' Institution

Department / Institution / Country
Kulliyyah Of Medicine & Health Sciences / Sultan Abdul Halim Mu'adzam Shah International Islamic University / Malaysia1
Department Of Parasitology, Faculty Of Medicine / University Of Malaya / Malaysia2
Content
Abstract Content

A protocol for water sample processing for the detection of Blastocystis sp. was previously established. The protocol was applied in 106 various water samples (31 river, 12 lake, 13 tap and 50 bottled drinking water). Briefly, 1.0 L water sample was collected and processed on the same day, centrifuged at 1400 x g for 10 min. For the isolation of Blastocystis sp. cysts, parasite pellet was on top of Ficoll-Paque™ PLUS, centrifuged at 1400 x g for 20 min and washed twice using 0.9% saline with centrifugation at 1400 x g for 10 min. Pellet was reconstituted in 1ml PBS. A minimum of 100 µl of the mixture was inoculated in 3 ml Jones’ medium supplement with 10% horse serum, incubated at 37°C and examined for any presence of vacuolar forms of Blastocystis sp. after 3 days of inoculation.  The results were presented as the percentage of occurrence of vacuolar forms of Blastocystis sp. using in vitro cultivation. The quality of water samples including physical parameters, faecal coliform counts and chemical content of water samples were determined. Blastocystis sp. was detected in 13.2% (14/106) of the water samples. The parasite was only detected in river samples (14/35, 45.2%), while this parasite was absent from all other water sources (lake, tap and bottled drinking water). It indicated that river samples were contaminated with faecal materials containing Blastocystis sp.  This concur with the quality of the water samples that contained high turbidity (60.89 NTU) were highly contaminated with faecal materials as indicated by the presence of Enterobacter sp. (2.87±2.7 colony X 106/ 100 ml water sample), Escherichia coli (1.19±1.10 colony X 106/ 100 ml water sample), ammonia (5.14±4.83 mg/ L water sample), nitrate (0.18±0.09 mg/ L water sample) and nitrite (0.075±0.047 mg/ L water sample).

Keywords: Blastocystis; cysts; detection; protocol; water
Requires Audio or Video system for Presentation?: No