MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

PHYSICAL, ENVIRONMENTAL AND CHEMICAL EFFECTS ON THE SURVIVAL OF BLASTOCYSTIS SP. CYSTS

Type
E-Poster Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Ii Li Lee1
Presenting Author
Ii Li Lee1
Co-Author
Tian Chye Tan2
Suresh Kumar Govind2

Authors' Institution

Department / Institution / Country
Kulliyyah Of Medicine & Health Sciences / Sultan Abdul Halim Mu'adzam Shah International Islamic University / Malaysia1
Department Of Parasitology, Faculty Of Medicine / University Of Malaya / Malaysia2
Content
Abstract Content

The survivability of Blastocystis spp. cysts in chlorine, salt water, pH and ultra violet light exposure, detergent and alcohol has yet to be elucidated and is important to assess the viability of Blastocystis sp. especially when subjected to the collection and detection protocols for this parasite. 

Physical parameters including storage temperature and duration, environmental parameters such as salinity, pH, UVC light and chlorine; and chemical parameters including 7X cleaning solution and Bactol™ alcohol gel were assessed.

Results indicated a significant reduction was observed in the viability of Blastocystis sp. cysts from 100.0% on day 0-41.6 % on day 7 at room temperature and 4°C in water samples. A sharp decrease in the percentage of viable Blastocystis sp. cysts was observed at 2 and 3.5% of salinity, where only 15.2±2.4% and 16.0±0.9% of viable cysts were detected, respectively. The parasites survived alkaline pH with a significant decrease in viability (p<0.01) 72.5±3.7% (pH 7.42) to 52.3±1.8% (pH 9.07) and 43.7±6.7% (pH 11.00). A significant (p<0.05) decrease of viability in Blastocystis sp. cysts (64.0±3.2%-43.7±4.9%) after exposure of UVC light for 24hr. There was no significant difference observed when exposed to calcium hypochlorite. The percentage of viable cysts ranged from 45.9-54.4% as compared to control (67.2±3.2%). Cleaning solution 7X affected the viability of Blastocystis sp. cysts significantly at p<0.01 as compared to control (64.9±7.8%). At 2.5, 5.0 and 10.0%, only 12.8±0.0%, 13.9±1.8% and 5.6±0.8% of Blastocystis sp. cysts were still viable respectively. Blastocystis sp. cysts was significantly reduced from 67.2±3.2% to 59.7±1.8% (p<0.05), 41.6±3.2% (p<0.01), 39.5±1.8% (p<0.01) and 31.9±3.1% (p<0.001) after exposure to Bactol™ alcohol gel.

In conclusion, this study implied that the cysts of Blastocystis sp. are resistant and robust. Therefore, the results must be considered when developing collection and detection protocols of Blastocystis sp. from water sources.   

Keywords: Blastocystis; water; chlorine; salinity; pH
Requires Audio or Video system for Presentation?: No