MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

IN-VITRO AND IN-VIVO STUDY: AN ALTERNATIVE TREATMENT TO COMBAT BLASTOCYSTIS SP. INFECTION

Type
Oral Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Sonal Girish 1
Presenting Author
Sonal Girish 1
Co-Author
Prof. Dr Suresh Kumar Govind1
Associate Prof. Dr. Norhaniza Aminudin2

Authors' Institution

Department / Institution / Country
Parasitology / University Malaya / Malaysia1
Institute of Biological Sciences / University Malaya / Malaysia2
Content
Abstract Content

Blastocystis sp. infection, although many remain asymptomatic, there is growing data in recent studies that suggests it is a frequent cause of gastrointestinal symptoms in children and adults. This proposes that treatment against this infection is necessary. Metronidazole (MTZ) which is the current choice of treatment, has expressed non-uniformity in its efficacy in combating this infection which has led to the study of alternative treatment. This study aimed to screen for Malaysian herbal extracts for its therapeutic potential and Eurycoma longifolia, Tongkat Ali (TA) exhibited the highest in-vitro anti-blastocystis activity. The aqueous extract demonstrated growth inhibition of 94.5%, 94.4% and 93.6% across ST1, ST3 and ST5 isolates respectively which was comparable to that of the reference drug, MTZ. This in-vitro outcome was further extrapolated to in-vivo where 24 Sprague Dawley (SD) rats, females and males, were tested upon for the assessment of the anti-protozoal activity effectiveness between TA and MTZ against ST3 isolate of Blastocystis sp. TA, in both female (86.2%) and male (84.4%) rats, exhibited statistically significant (p<0.05) reduction in the number of cyst/ml count of Blastocystis sp. compared to control. MTZ on the other hand while exhibited statistically significant (p<0.05) reduction in the number of cyst/ml count of Blastocystis sp. in female rats (65.1%), in male rats however the reduction was insignificant (p>0.05) with 40.1% compared to control. These findings highlighted the potential therapeutic effect of TA as a more uniformed, comparable and natural alternative treatment against Blastocystis sp. infection.

Keywords: Allopathic drugs; Tongkat Ali; drug susceptibility assay; subtypes
Requires Audio or Video system for Presentation?: No