MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

IN VITRO AND EX VIVO THERMAL STRESS INDUCES PROLIFERATION OF BLASTOCYSTIS SP.

Type
E-Poster Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Gaythri Thergarajan1
Presenting Author
Gaythri Thergarajan1
Co-Author
Suresh Kumar Govind1
Subha Bhassu

Authors' Institution

Department / Institution / Country
Parasitology / University of Malaya / Malaysia1
Content
Abstract Content

Blastocystis sp. being the common eukaryotic inhabitant of the human intestine has been always regarded as a commensal with recent studies implicating it to be a parasite with a pathogenic potential.  Binary fission has been widely accepted as plausible mode of reproduction for this parasite. The present study demonstrates that when subjecting the parasites in vitro to higher temperature the parasite shows proliferation in numbers. Transmission electron microscopy was used to compare the morphology of Blastocystis sp. subtype 3 isolated from a dengue patient having high fever (ex vivo thermal stress) and Blastocystis sp. 3 maintained at 41°C (in vitro thermal stress) and 37°C (control). Blastocystis sp. at 37°C were found to be mostly vacuolar whereas the in vitro thermal stressed isolates at 41°C were granular with electron dense material seen to protect the granules within the central body. Parasites of the ex vivo thermal stressed group showed similar ultrastructure as the in vitro ones. Upon exposure to high temperature both in vitro and ex vivo, Blastocystis sp. in cultures showed higher number of granular forms. These forms were seen to be protected by the electron dense material within the central body possibly acting as a protective mechanism. This is possibly to ensure the ability to survive for the granules to be developed as viable progenies for release into the host system.

Keywords: Blastocystis sp.; thermal stress; granules;
Requires Audio or Video system for Presentation?: No