MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

STUDIES TO ELUCIDATE THE GENES RESPONSIBLE FOR STRESS RESPONSE AND GROWTH IN BLASTOCYSTIS SP.

Type
Oral Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Gaythri Thergarajan1
Presenting Author
Gaythri Thergarajan1
Co-Author
Suresh Kumar Govind1
Subha Bhassu

Authors' Institution

Department / Institution / Country
Parasitology / University of Malaya / Malaysia1
Content
Abstract Content

Metronidazole (MTZ) is given as the first line treatment upon diagnosis of Blastocystis sp. infection. MTZ has been shown to trigger apoptotic mechanism which also facilitate the formation of granular forms. At the same time, Blastocystis sp. was also reported in vitro and ex vivo to proliferate in numbers when responding to thermal stress by producing granular forms. In the present study, an attempt is made to observe the effect of MTZ treatment on biochemical changes like apoptosis on the in vitro and ex vivo thermal stressed Blastocystis sp. Thermal stressed Blastocystis sp, when further induced with 0.001 mg/ml metronidazole (MTZ), high number of apoptotic forms were formed. Apoptotic forms which were granular release progenies at 72 hours increasing the number of viable parasites. Metronidazole or drug stress triggers the formation of apoptotic cells that are granular in nature. The granules matured and was released which accounted for the higher parasite number at 72 hours. These phenotypic changes were also well associated with genotypic expression. HSP70, a stress response gene was found to up regulate when Blastocystis sp. underwent thermal and drug stress. This could possibly facilitate the protection against those stresses to the parasite at the same time allow proliferation of the parasite within the mother cell. On the other hand, the SIR2, a gene associated to apoptotic mechanism were up regulated when thermal stressed parasite exposed to MTZ. This could possibly be due to the formation of granular type apoptotic forms. HSP70 and SIR2 genes have shown higher expression to enhance the formation of reproductive type granular forms which will then be released as viable parasites. Both the genotypic and phenotypic observations were found to have strong relationship in ensuring the survival of Blastocystis sp.

Keywords: Blastocystis sp.; HSP70; SIR2; granular forms
Requires Audio or Video system for Presentation?: No