MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

MONOCYTES RESPONSE DURING ACUTE AND CHRONIC PHASES OF BLASTOCYSTIS SP. ST3 INFECTIONS

Type
E-Poster Presentation Competition for Student
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Sheela Devi Sugadan1
Presenting Author
Sheela Devi Sugadan1
Co-Author
Chandramathi Samudi2
Suresh Kumar Govind 1

Authors' Institution

Department / Institution / Country
Parasitology / University of Malaya / Malaysia1
Microbiology / University of Malaya / Malaysia2
Content
Abstract Content

Blastocystis sp., an intestinal eukaryotic parasite reported to disrupt the intestinal barrier integrity, alter the immune homeostasis of the intestinal regions and colonise the host through several mechanisms. This study aimed to evaluate the monocytes trafficking through 1) pro-inflammatory and anti-inflammatory cytokine secretions and 2) cell activation and differentiation; modulated by asymptomatic and symptomatic isolates of Blastocystis sp. ST3 antigen during acute/short term (3 days) and chronic/long term (6 days) infection. Monocytes cell line was induced with 3 different isolates of symptomatic and asymptomatic Blastocystis sp. ST3 at short term (3 days) and long term (6 days).  Cell proliferation, cytokine analysis and Programme Cell Death-1 (PD-1) expression were assessed. Symptomatic and asymptomatic isolates proliferated at mean ± SD of (1807570±140468) and (1943894±113350) respectively during short term induction and (2119340±193979) and (1666789±147155) during long term induction. During short term assay, symptomatic induced group significantly secreted higher IL-6 (397±158) and IL-10 (18.4±3.7) cytokines compared to the non induced control group (p<0.001) whereas asymptomatic isolates secreted higher IL-12p70 (12±8) and TNFα (183±12) when compared to non-induced control group (P<0.001). Long term assay exhibited an opposite cytokine secretions where the IL-6, TNFα and IL-10 levels in the asymptomatic isolates were higher than symptomatic isolates with (502±194), (697±140) and (443±21)  respectively compared to the non induced control group (P<0.001). In the other hand, symptomatic isolates secreted higher IL-12p70 (334±34) when compared to non induced control group (P<0.001). The PD-1 marker increased significantly p<0.05 from short to long term proliferation assay in both symptomatic and asymptomatic isolates with the percentage of (17% to 29%) and (14% to 28%) respectively. This study proved that, ST3 from symptomatic and asymptotic individuals modulates an opposite immune response during acute and chronic phase of infections which leads to different innate immune evasion pathways.

Keywords: Blastocystis sp., subtype 3, monocytes, immune modulation
Requires Audio or Video system for Presentation?: Yes