MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

DIFFERENTIAL IMMUNE RESPONSE INDUCED BY SYMPTOMATIC AND ASYMPTOMATIC ISOLATES OF BLASTOCYSTIS SP. SUBYTPE 3

Type
Oral Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Medical Microbiology & Parasitology

Authors

Main Author
Sheela Devi Sugadan1
Presenting Author
Sheela Devi Sugadan1
Co-Author
Chandramathi Samudi2
Suresh Kumar Govind1

Authors' Institution

Department / Institution / Country
Parasitology / University of Malaya / Malaysia1
Microbiology / University of Malaya / Malaysia2
Content
Abstract Content
Blastocystis sp. subtype 3 (ST3) has been implicated previously to be phenotypically heterogeneous between isolates from symptomatic and asymptomatic individuals. The aim of this study is to identify the immunogenic and antigenic differences among three different isolates of Blastocystis sp. ST3 isolated from asymptomatic and symptomatic individuals. Splenocyte proliferation, Th1/Th2 cytokine responses and IgG/IgG1/IgG2a antibodies were assessed. Total IgG antibody responses in sera from mice immunised with symptomatic and asymptomatic groups were (0.426±0.025) and  (0.736±0.033) respectively, significantly higher compared to PBS control (P<0.05). Mixed IgG1/IgG2a response with predominant IgG1 secretion in sera from asymptomatic group (0.148±0.016) and predominant IgG2a secretion in sera from symptomatic group (0.355±0.021) compared to PBS control (P<0.05). This data further supported by high Th1 (IL-2 and IFNγ) cytokine response in symptomatic group (628.89±31.06) and (393.56±49.21) ; high Th2 (IL-4 and IL-10) cytokine response (93±7.85) and (103.22±11.33) in asymptomatic group after in vitro splenocyte stimulations. Proliferation of stimulated splenocyte was significantly high in both the groups compared to PBS control. The present results show the first time that, distinct immunological response were mediated by ST3 symptomatic and asymptomatic Blastocystis sp. by modulating the different rate of lymphocytes cells proliferation and Th1/Th2 cytokine immune response. Therefore, this study has proved that symptomatic isolates are highly immunogenic and antigenic compared to asymptomatic isolates with distinct immunological response among the same subtype.
Keywords: Blastocystis sp., subtype 3, immunization, asymptomatic, symptomatic
Requires Audio or Video system for Presentation?: Yes