MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

PHYLOGENETIC ANALYSIS OF HEMAGGLUTININ GENE OF HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUS ISOLATED FROM RECENT OUTBREAK IN SABAH, MALAYSIA IN 2018

Type
E-Poster Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Emerging Zoonoses

Authors

Main Author
Leow Bee Leng1
Presenting Author
Leow Bee Leng1
Co-Author
Syamsiah Aini Shohaimi1
Faizul Fikri Mohd Yusop1
Muhammad Redzwan Sidik1
Geok Huai Ong1
Faizah Hanim Mohd Saied1

Authors' Institution

Department / Institution / Country
Depertment of Veterinary Services / Institut Penyelidikan Veterinar / Malaysia1
Content
Abstract Content

Highly Pathogenic Avian Influenza (HPAI) is a highly contagious disease in poultry where outbreaks can cause flock mortality up to 100% in two to three days. It has been one of the major causes of economic losses in the poultry industries. In July 2018, high mortality rate of layer chickens in a commercial layer farm in Kampung Kauluan, Tuaran, Sabah were reported. The causative agent was then identified as Avian Influenza H5N1 virus through both virus isolation and molecular detection. BLAST analysis indicated that the nucleotide of the hemagglutinin (HA) gene of the isolate was 98% similar with the China H5N1 isolate. Phylogenetic analysis revealed that the isolate belonged to the clade 2.3.2.1c. The presence of multi-basic amino acid motif, the PQRERRRKR/GLF at the cleavage sites of the HA protein indicate that the isolate was a HPAI virus. The virus also possess a conserved glutamine (Q) and glycine (G) at position 222 and 224 (H5 numbering) respectively, indicating that the virus retains the avian receptor binding preference. This was the first HPAI H5N1 outbreak in Sabah. A total of seven villages were affected involving commercial layer chickens, village chickens and ducks. In addition, this was the second reported H5N1 clade 2.3.2.1c outbreak in Malaysia with the first outbreak occurred in Kelantan back in 2017. The introduction of this virus into Sabah and East Malaysia for the first time raised an alert alarm regarding the potential of this virus becoming an epidemic problem. High farm biosecurity, continuous surveillance programme, accurate rapid laboratory diagnostic capabilities and risk assessment for this virus with pandemic potential are needed as prevention steps and preparation in dealing with new and emerging influenza viruses in the country.

 

 

Keywords: HPAI; H5N1; clade 2.3.2.1c; Sabah
Requires Audio or Video system for Presentation?: No