MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

MICROSCOPIC DETECTION OF SARCOCYSTIS IN SLAUGHTERED GOAT IN TAPAH, PERAK

Type
E-Poster Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Veterinary Parasitology

Authors

Main Author
Fazly Ann Zainalabidin1
Presenting Author
Fazly Ann Zainalabidin1
Co-Author
Muhamad Syamsul Naim Noor Azmi1
Niny Fariza Junoh1
Wan Normaziah Wan Omar Bakri1
Geethamalar Sathayah1

Authors' Institution

Department / Institution / Country
Department of Veterinary Services / Veterinary Research Institute / Malaysia1
Content
Abstract Content

Sarcocystis spp. is a cyst forming coccidia with an obligatory two-host life cycle. The parasites infect many species of livestock including goats. The most common species found in goats during necropsy were S. capracanis and S. hircicanis. The purpose of this study was to determine the frequency of sarcocystis infection in slaughtered goat by digestion technique and histological examination. Ninety fresh muscle samples consists of heart (n1=30), oesophagus (n2=30), and thigh muscle (n3=30) from 30 mix breed of goats were randomly collected from Tapah abattoir commencing from March to August 2016. All samples were grossly observed for macroscopic cyst prior dividing it into two parts for further laboratory microscopic detection. A part of the samples were stored at 4° to 6°C for sarcocyst bradyzoites detection by digestion technique. Another part of the samples were transported in 10% formalin before being processed and examined under the light microscope to detect the presence of sarcocyst by histological examination. Out of 30 animals, 7 (23.3%) animals were detected positive with microscopic Sarcocystis spp. with 5 (71.43%) animals were diagnosed from thigh muscle samples and 1 (14.29%) animal each were diagnosed from heart, and combination of heart, oesophagus and thigh muscle samples, respectively. For the digestion technique, 57.14% (4 of 7) of thigh muscle samples and 28.57% (2 of 7) of heart muscle samples from positive animals were detected with sarcocyst bradyzoites. Histological examination revealed that, 28.57% (2 of 7) of thigh muscle samples and 14.29% (1 of 7) each of heart and oesophagus samples were diagnosed positive with microscopic sarcocyst, respectively. Based on the results, almost quarter of the slaughtered goats were diagnosed positive with sarcocystis by both microscopic methods. However, limitation to identify the cyst until the species level need to be taken into consideration when applying this conventional microscopic methods.

Keywords: digestion technique, goat, histology, sarcocystis, slaughter
Requires Audio or Video system for Presentation?: No