MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

INTERLEUKIN-35 (IL-35) MODULATION ALTERS CYTOKINES ENVIRONMENT, HISTOPATHOLOGICAL FEATURES AND SURVIVAL OF P. BERGHEI INFECTED MICE

Type
E-Poster Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Malaria

Authors

Main Author
Ramatu Bello1
Presenting Author
Rusliza Basir1
Co-Author
Maizaton Atmadini Abdullah1
Roslaini Abd Majid1
Khairi Hussain1
Mohammed Faruq Isnadi1
Zaid Ibraheem1
Rusliza Basir1

Authors' Institution

Department / Institution / Country
Faculty of Medicine & Health Sciences / Universiti Putra Malaysia / Malaysia1
Content
Abstract Content

A comprehensive understanding of the interaction between malaria parasite(s) and the immune system is very important in the development of drug therapies and vaccines againts the disease. The balance between pro- and anti-inflammatory cytokines represent crucial determinants influencing the onset of protective or non-protective responses to malaria infection. IL-35 has been reported to exert beneficial anti-inflammatory effects in many disease conditions. Here, we investigated the effects of modulating IL-35 in mice infected with P. berghei.

Juvenile male ICR mice inoculated with P. berghei infected red blood cells were utilized for this study. Following infection, mice received either recombinant-IL-35 protein, neutralizing antibody against IL-35, Immunoglobulin-G or phosphate buffered saline respectively. Drug treatments and parasitemia estimates were performed daily for a duration of 4 days after initiation of infection following which serum and organs were harvested from the animals for cytokine measurement using flow cytometry and also histopathological assessment.

Cytometric bead array analysis revealed that neutralizing IL-35 enhanced the release of IFN-γ and IL-10 significantly, whereas augmenting IL-35 enhanced IL-6 release. Furthermore, IL-35 neutralization significantly improved survival rate and histopathological features of severe infection in various organs whereas augmentation of IL-35 produced noticeable decrease in survival and failed to improve histopathological features of illness. The beneficial influence of IL-35 neutralization on malaria infected mice may be attributed to IFN-γ and IL-10 release. IFN-γ has been reported to involve in parasite clearance hence its capacity to ameliorate malaria disease severity, positively impact histopathological indices and prolonged survival. IL-10 is anti-inflammatory in nature and may help balanced the effects of pro-inflammatory cytokines release during malaria infection.

In conclusion, neutralization of IL-35 may offers potential immunotherapeutic benefit which can be considered as an important target for the amelioration of severe malaria infection as well as for vaccine development.

Keywords: Malaria, Interleukin-35, Plasmodium berghei
Requires Audio or Video system for Presentation?: No