MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

LARVICIDAL EFFICACY OF SARAWAK PIPER NIGRUM L. (PIPERACEAE) ETHANOLIC EXTRACT AGAINST DENGUE VECTOR, AEDES ALBOPICTUS (DIPTERA: CULICIDAE) AND ITS PHYTOCHEMICAL CONSTITUENTS

Type
E-Poster Presentation Competition for Student
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Vector Biology & Control

Authors

Main Author
Emira Izzati Abdul Aziz1
Presenting Author
Emira Izzati Abdul Aziz1
Co-Author
Nor Aliza Abdul Rahim1
Siti Zaleha Raduan1

Authors' Institution

Department / Institution / Country
Department of Paraclinical Sciences, Faculty of Medicine and Health Sciences / UNIVERSITI MALAYSIA SARAWAK (UNIMAS) / Malaysia1
Content
Abstract Content

Resistance development of vector mosquitoes towards chemical insecticides such as Temephos have been documented in Malaysia. Therefore, botanical derived insecticide may become an alternative biocontrol in controlling mosquito population. This study was conducted to determine the larvicidal efficacy of Sarawak Piper nigrum L. (Piperaceae) fruit and leaf ethanolic extracts against dengue vector, Aedes albopictus (Diptera: Culicidae) and its phytochemical constituents. Plant samples were extracted in 95% ethanol, evaporated using rotary evaporator and analysed using GC-MS. A total of fifty compounds were detected from P. nigrum L. extracts. The major compounds were piperidine (16.78%), caryophyllene (8.28%) and piperine (6.86%) from the fruit extracts, and furanol (17.51%), benzenethanamine (12.79%), caryophyllene (3.99%) and piperidine (3.20%) from the leaf extract. The crude extracts were also diluted into concentrations of 2.5-10.5ppm and 50-450ppm for fruit and leaf extracts, respectively. The larvicidal bioassays were conducted using crude extracts following World Health Organization (WHO) standard for larval susceptibility test. In this study, both P. nigrum extracts showed 100% larvae mortality at the highest concentration tested (10.5 ppm and 450 ppm) after 24 hours of exposure. The larvicidal efficacy between P. nigrum fruit and leaf extracts at 24-hours bioassay test was significantly different (F=11.854, df=1, p< 0.05). The P. nigrum fruit extract has shown a more remarkable larvicidal potential with lower concentration for LC50 (5.07 ppm) and LC90 (7.85 ppm) if compared to leaf extract which required higher concentration for LC50 (125.39 ppm) and LC90 (293.91ppm). Various phytochemical constituents in P. nigrum L. extracts may be responsible for the variability in their larvicidal potential and contribute synergistic effect against Ae. albopictus larvae. Therefore, the Piper nigrum L. from Sarawak deserved to be considered for further development due to its potential for Ae. albopictus mosquito biocontrol.

Keywords: Dengue vector; Aedes albopictus; Piper nigrum L.; larvicidal bioassays; GC-MS; phytochemicals
Requires Audio or Video system for Presentation?: No