MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

VECTOR STATUS AND TRANSOVARIAL TRANSMISSION OF CHIKV IN AEDES MOSQUITOES ON SALAK ISLAND, KUCHING, SARAWAK

Type
E-Poster Presentation Competition for Student
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Vector Biology & Control

Authors

Main Author
Cindy Tan Siew Ching1
Presenting Author
Cindy Tan Siew Ching1
Co-Author
Nor Aliza Abdul Rahim1
Anna Andrew1

Authors' Institution

Department / Institution / Country
Department of Paraclinical Sciences, Faculty of Medicine and Health Sciences / Universiti Malaysia Sarawak / Malaysia1
Content
Abstract Content

Chikungunya virus (CHIKV) is an alphavirus which can be transmitted through Aedes aegypti and Aedes albopictus. Transovarial transmission of CHIKV in vector mosquito occur when the virus is disseminated within the infected female mosquitoes’ ovarian cells to eggs during the process of fertilization. Transovarial transmission allow maintenance of the virus in the vector mosquito population. This study was conducted to determine the vector status and transovarial transmission of CHIKV in Aedes mosquitoes on Salak Island, Kuching, Sarawak, that experienced outbreak of CHIKV in 2009. Mosquito larvae were collected by using ovitraps and from discarded receptacles around the village while adult mosquitoes were sampled by using mechanical aspirator.  Emerged and field caught adult Aedes mosquitoes were pooled before subjected to RNA extraction and two-step reverse transcriptase polymerase chain reaction (RT-PCR). The positive pools were further analyzed for genotypes. The ovitrap index recorded was 84.3% and 96.7% for the first and second sampling respectively. The species of mosquitoes collected in the village was Aedes albopictus (88.79%) followed by Aedes aegypti (6.32%), Culex sp. (3.20%) and Armigeres sp. (1.56%). CHIKV was positive in two pools (n=35) from emerged adults Ae. albopictus; one male and one female pool. The CHIKV detected belongs to the East Central South African genotypes. None of the adult field-caught Aedes mosquitoes were positive for CHIKV. This study showed a high occurrence of CHIKV vectors on the island, evidence of its transovarial transmission in Aedes albopictus and its genotype. The information gained may serve as a reference for future vector surveillance and in situation if another outbreak occurs.

Keywords: Transovarial transmission; Aedes mosquitoes; RT-PCR ; chikungunya virus; East Central South African genotypes.
Requires Audio or Video system for Presentation?: No