MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

SCREENING OF NATURAL WOLBACHIA INFECTION IN AEDES MOSQUITOES USING MAY-GRÜNWALD-GIEMSA STAINING

Type
E-Poster Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Vector Biology & Control

Authors

Main Author
Aishah Hani Azil1
Presenting Author
Aishah Hani Azil1
Co-Author
Nurul Atira Norizan2
Fatin Liyana Shahabudin2
Wong Mann Ru2
Addeena Nurliyana Roka Rosam2
Muhammad Zaid Mohd Firdaus2
Noor Shazleen Husnie Mohd Mohtar1
Ahmad Firdaus Mohd Salleh1

Authors' Institution

Department / Institution / Country
Department of Parasitology & Medical Entomology / Faculty of Medicine, Universiti Kebangsaan Malaysia / Malaysia1
Medical Undergraduate / Faculty of Medicine, Universiti Kebangsaan Malaysia / Malaysia2
Content
Abstract Content

Wolbachia pipientis is known to persist naturally in Aedes albopictus through vertical transmission (i.e. mother to offspring), unlike Aedes aegypti as shown in most studies. The objective of the study was to detect the presence of Wolbachia in Aedes mosquito population at a residential college in Cheras, Kuala Lumpur using May-Grünwald-Giemsa staining. Mosquito collections were performed both indoors and outdoors using standard ovitraps. Aedes eggs were then brought to the insectarium to be reared into adults. Female Aedes mosquitoes were dissected for their ovaries and midguts. The tissues were heat fixed on glass slides and stained with May-Grünwald-Giemsa before microscopic examination was performed. Next, the samples were compared with positive control slides which were confirmed by PCR. Out of 236 Aedes albopictus females, 39.4% were found to harbour Wolbachia. No Wolbachia was found in Aedes aegypti females but absence of natural infection in the species cannot be concluded due to small number of samples. The morphological features were easily identified as the bacteria had absorbed the May-Grünwald-Giemsa stain well. The rod-shaped microorganisms, ranging from 1.31 to 1.95 µm in length, had a dark purple hue and can be clearly seen on a pale purple background. The morphological features of Wolbachia in Drosophila simulans was similar with the exception of it having a wider range in size (1.1 to 2.56 µm in length) indicating a different Wolbachia strain or phenotype. Results from this study substantiate the use of May-Grünwald-Giemsa staining for screening Wolbachia infection in mosquitoes.

Keywords: Wolbachia; Aedes; May-Grünwald-Giemsa; staining;
Requires Audio or Video system for Presentation?: No