MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur




E-Poster Presentation Competition for Student
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Vector Biology & Control


Main Author
Mohd Farihan Md Yatim1
Presenting Author
Mohd Farihan Md Yatim1
Nazarudin (Dr) Safian2
Ahmad Firdaus Mohd Salleh1
Aishah Hani (Dr) Azil1

Authors' Institution

Department / Institution / Country
Parasitology and Medical Entomology, Faculty of Medicine / Universiti Kebangsaan Malaysia / Malaysia1
Community Health, Faculty of Medicine / Universiti Kebangsaan Malaysia / Malaysia2
Abstract Content

The use of attractant(s) in ovitraps is often needed to outcompete natural breeding containers. Several studies suggested presence of ammonia in water could attract Aedes aegypti mosquito to oviposit. However, no specific study was done to measure oviposition attractancy of ammonia-based solution. In this study, three ammonia-based solutions were evaluated using laboratory bioassays in 35 x 35 x 35 cm cage. Formulation 1 was aqueous ammonia of three different concentrations (1 ppm, 2.5 ppm and 5 ppm). The other two ammonia-based formulations used were 2 ppm ammonium nitrate (Formulation 2) and 12.75 ppm ammonium with 1.20 ppm lactic acid (Formulation 3). Both formulations derived from previous studies in which Formulation 2 was detected in Aedes aegypti infested public drinking water in Brazil and Formulation 3 was from phytochemical analysis of 10% hay infusion. 30 replicates were performed for all formulations and Oviposition Activity Index (OAI) was used to measure attractancy value.  1 ppm aqueous ammonia averaged eggs collection was 171.60 ±65.36 compared to control 147.97±83.20 with OAI value of +0.07, which was the highest compared to the 2.5 ppm and 5 ppm. Averaged eggs collection of Formulation 2 was 134.13±56.50 compared to control 116.13±63.07 with OAI value of +0.07. Formulation 3 collected 152.87±60.58 compared to control 123.77±63.83 with OAI value of +0.11. However, t-Test analysis for all three formulations showed no significant difference compared to control with p values of p=0.226, p=0.249 and p=0.075 for Formulation 1, 2 and 3 respectively.  Though, based on the OAI value, it was concluded that all ammonia-based solutions tested are weak oviposition attractants for Aedes aegypti, with formulation of 12.75 ppm ammonium with 1.20 ppm lactic acid being the most attractive.

Keywords: oviposition attractant; aqueous ammonia; ammonium nitrate; lactic acid; aedes aegypti
Requires Audio or Video system for Presentation?: Yes