MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

ERTAPENEM NON-SUSCEPTIBILITY TEST IN PREDICTING NDM-1 AND IMP-1 PRODUCTION IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES

Type
E-Poster Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Antibiotic Resistance

Authors

Main Author
Salman Ghaffari2
Presenting Author
Salman Ghaffari2
Co-Author
Zahra Shahandeh3
Narges Kalantari1
Farahnaz Sadighian3

Authors' Institution

Department / Institution / Country
Cellular and Molecular Biology Research Center, Health Research Institute / Babol University of Medical Sciences / Iran, Islamic Rep. of1
Department of Mycology and Parasitology / Babol University of Medical Sciences / Iran, Islamic Rep. of2
Department of Laboratory Sciences / Babol University of Medical Sciences / Iran, Islamic Rep. of3
Content
Abstract Content

Introduction: Carbapenem-resistant Enterobacteriaceae (CRE) isolates are resistant to ertapenem, doripenem, imipenem and meropenem have been reported throughout world.  They cause serious problems in the treatment of infectious diseases as they hydrolyze carbapenems leading to treatment failure. These isolates carry various metallo-β-lactamases genes and a routine phenotypic test has not been suggested. This study aimed to compare the ertapenem non-susceptibility test with polymerase chain reaction in predicting the production of MBLs in E. coli.

Methods and materials: Antimicrobial susceptibility test for beta-lactam antibiotics were performed for isolated E. coli from clinical samples using disk diffusion method. All isolates with inhibition zones of ≤ 22 mm for CAZ and ≤ 27 mm for CTX were considered possible MBLs producing isolates. The production of metallo-β-lactamases (MBLs) was evaluated by non-susceptibility to ertapenem and a conventional PCR. DNA was extracted from the bacterial suspension using a commercial DNA extraction kit according to manufacturer's instructions. PCR analysis of  blaIMP-1 and blaNDM-1 genes was performed using a specific primer pair.

Results: Out of 259, 138 (53.3%) isolates were potential MBLs producing bacteria and 115 out of 138 isolates were susceptible to ertapenem. The blaNDM-1 or/and blaIMP-1 genes were found in 39/115(33.9%) isolates which were susceptible to ertapenem. The sensitivity of ertapenem non-susceptibility test compared with PCR was 15.2%, and the specificity was 82.6%.

Conclusion: The findings obtained here showed that the E. coli isolates which are susceptible to ertapenem should be further evaluated for carbapenemase production by PCR methods.

Keywords: E. coli; Ertapenem; blaNDM-1; blaIMP-1
Requires Audio or Video system for Presentation?: No