MSPTM 2019 Annual Scientific Conference
13 - 14 March 2019
InterContinental Kuala Lumpur

Abstract

Title

NEW DELHI METALLO-BETA-LACTAMASE-1 PRODUCING ESCHERICHIA COLI: A FIRST MOLECULAR IDENTIFICATION AND CHARACTERIZATION OF EXTRAINTESTINAL AND ENTERIC STRAINS ISOLATED FROM PATIENTS IN NORTH OF IRAN

Type
E-Poster Presentation
Theme
Scaling Up Efforts in Tropical Disease and Vector Control through Evidence-Based Research
Topic
Antibiotic Resistance

Authors

Main Author
Narges Kalantari4
Presenting Author
Narges Kalantari4
Co-Author
Zahra Shahandeh3
Farahnaz Sadighian3
Mohammad Etminan1
Salman Ghaffari2
Hourieh Aram3

Authors' Institution

Department / Institution / Country
Student Research Committee / Babol University of Medical Sciences / Iran, Islamic Rep. of1
Mycology and Parasitology / Babol University of Medical Sciences / Iran, Islamic Rep. of2
Department of Laboratory Sciences / Babol University of Medical Sciences / Iran, Islamic Rep. of3
Cellular and Molecular Biology Research Center, Health Research Institute / Babol University of Medical Sciences / Iran, Islamic Rep. of4
Content
Abstract Content

Introduction: Carbapenemases producing bacteria, particularly metallo beta-lactamases (MBLs), cause serious problems for the treatment of infectious diseases since they are usually highly resistant to most β-lactams and are considered to be multi-drug resistant (MDR).  The new Delhi metallo beta-lactamase-1 (NDM-1) is a MBLs found in E.coli.  NDM-1 producing strains are very hazardous as the genes are located on plasmids, therefore transfer quickly and become widespread around the world.  In the present study, we aimed to detect and characterize NDM-1 expressing E.coli, in addition to evaluating the frequency of  blaCTX-M, blaTEM-1&2, blaSHV-1, and blaIMP-1 genes among E. coli, isolated from clinical samples. 

Methods: Susceptibility to antibiotics was performed by disc diffusion method. ESBLs and  metallo-beta-lactamase isolates were evaluated for blaCTX-M, blaNDM-1 and blaTEM-1&2, blaSHV-1, and blaIMP-1 genes using a conventional PCR method.

Results: Of 117 non-duplicate isolated E. coli, ESBL and carbapenemase were detected in 110 and 72 isolates, respectively. PCR analysis showed that the predominant beta-lactamase genes were blaCTX-M (95.5%), blaTEM-1&2 (43.6%) followed by blaNDM-1 (31.9%).  Out of 23 NDM-1 positive isolates, the production of carbapenemase and ESBLs were recognized in 23 and 22 isolates by phenotypic methods, respectively. BlaCTX-M, blaTEM-1&2 and blaIMP-1 genes were also detected in 95.7%, 26.1% and 8.7% of the NDM-1 positive isolates, respectively. Twenty-two isolates (95.7%) were resistant to aztreonam in addition to other antibiotics, and 21 isolates were susceptible to ertapenem.

Conclusion: To the best of our knowledge , this is the first report of  NDM-1 producing-E.coli isolates from the north of Iran. All NDM-1 producing isolates were MDR which is a potential health care threat and creates an important challenge for the treatment and control of infectious diseases. Furthermore, almost all E. coli isolates from clinical samples in our region carried the BlaCTX-M gene.

Keywords: E. coli; blaNDM-1; blaTEM; blaSHV; blaIMP, Iran
Requires Audio or Video system for Presentation?: No