|
Title Milk Fat Globule Membrane Alone and in Combination with a Prebiotic Blend Moderates the Impact of Maternal Separation on Behavior and Gut Microbiota
|
|
Type Poster Presentation |
|
Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
|
Topic Translational Microbiome Studies |
|
Main Author Sarmauli Manurung6 |
|
Presenting Author Sarmauli Manurung6 |
|
Co-Author Siobhain O'Mahony1 2 Karen-Anne McVey Neufeld1 Rosaline V. Waworuntu5 Matteo M. Pusceddu1 Kiera Murphy3 Conall R. Strain3 Catherine Stanton1 3 Brian M. Berg5 Timothy G. Dinan1 4 John F. Cryan1 2 |
|
Department / Institution / Country APC Microbiome / University College Cork / Ireland1 Anatomy and Neuroscience / University College Cork / Ireland2 Food Research Centre / Teagasc / Ireland3 Psychiatry and Neurobehavioural Science / University College Cork / Ireland4 Global Discovery / Mead Johnson Pediatric Nutrition Institute / United States5 Global Discovery / Mead Johnson Pediatric Nutrition Institute / Netherlands6 |
|
Background and Rationale Nutritional interventions targeting the microbiota-gut-brain axis was proposed to modulate stress-induced dysfunction of physiological processes and brain development.
|
|
Objectives: Indicates the purpose of the study To assess the effects of dietary supplementations through various nutritional interventions: milk fat globule membrane (MFGM) and a polydextrose/galactooligosacchardie prebiotic blend in maternally separated (MS) rats.
|
|
Methodology: Describe pertinent experimental procedures The MS rats were separated from their mothers for 3 h /day from postnatal day (PND) 2 to 12. Starting at weaning, both non-separated (NS) and MS offspring were provided with or without supplementation of MFGM, prebiotic blend or a combination of both. Spatial memory, visceral sensitivity and stress reactivity were assessed in adulthood. Gene transcripts associated with cognition and stress were measured and the caecal microbiota composition was analysed. |
|
Results: Summarize the results of the research MS rats demonstrated visceral hypersensitivity which was ameliorated by MFGM and the combination of MFGM+prebiotic. MS rats received prebiotic, MFGM alone, as well as the combination showed improved cognitive performance in spatial and reference memory. Coinciding with these, plasma corticosterone was higher in MS rats following the acute stress and was reduced by prebiotic and the combination, suggesting moderation of stress reactivity. Moreover, MFGM and prebiotic supplementation alone, and in combination, induced changes in microbiome composition of MS rats. Peptostrepococcaceae was increased in the prebiotic+MFGM group compared to the control group. The relative abundance of Barnesiella was increased with MFGM supplementation in comparison with control and prebiotic groups. Clostridium cluster IV was increased in prebiotic+MFGM compared to MFGM and control. Finally, MFGM and prebiotic supplementation alone, and in combination, had a significant effect on beta diversity, with a more profound effect in MS animals compared to NS animals. |
|
Conclusions: State the main conclusions Given the impact of MS on the gut microbiota and behavior was modulated by MFGM and the probiotic blend, these dietary supplementations may offer a nutritional solution to early-life stress induced alterations in the microbiota-gut-brain axis. |