Abstract

Title

Bioconversion of Ginsenoside Rb1 to Rd using β-Glucosidase from Leuconostoc Mesenteroides EFEL 15

Type
Poster Presentation
Theme
Probiotics and Prebiotics: Excellence in Science and Clinical Translation
Topic
Metabolic By-products of Probiotic Strains

Authors

Main Author
Ji Soep Shin1
Presenting Author
Ji Soep Shin1
Co-Author
Nam Soo Han1

Authors' Institution

Department / Institution / Country
Division of Animal, Horticultural, and Food Sciences / Chungbuk National University / Korea, Rep. (대한민국)1
Content
Background and Rationale
Ginsenoside Rd is a protopanaxadiol type ginsenoside having tetracyclic terpene sapogenin structure and it is known to exert diverse health promoting activities such as wound healing, immune modulation, anti-obesity, and managed mitochondrial function.
Objectives: Indicates the purpose of the study
We isolated a lactic acid bacterium from Kimchi showing high β-glucosidase activity and constructed a microbial conversion process to produce ginsenoside Rd from ginsenoside Rb1 by using the isolate.
Methodology: Describe pertinent experimental procedures
Lactic acid bacteria were isolated from Kimchi and their β-glucosidase activities were tested. Whole cells and lysed cells were used as the crude enzyme in the bioconversion reactions. The lysed cells were prepared by sonication and the bioconversion of Rb1 to ginsenoside Rd was carried out at 30°C for 72 hrs. The strain was identified by phenotypic and genotypic analyses.
Results: Summarize the results of the research
As result, Leuconostoc mesenteroides EFEL 15 strain was isolated as a potential strain. When the whole cells and lysed cells were reacted with ginsenoside Rb1 in an optimized condition, ginsenoside Rd was produced after 24 hrs. TLC and HPLC analyses showed that the enzyme extract and whole cells hydrolyzed 20-C-β-(1→6)-glucoside linkage of Rb1 compound.
Conclusions: State the main conclusions
Leuconostoc mesenteroides EFEL 15 strain can be used for overproduction of ginsenoside Rd in the ginseng-derived fermented food.
Keywords: Bioconversion, Ginsenoside Rb1, Ginsenoside Rd, β-Glucosidase, Leuconostoc mesenteroides
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