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Title Bioconversion of Ginsenoside Rb1 to Rd using β-Glucosidase from Leuconostoc Mesenteroides EFEL 15 |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Metabolic By-products of Probiotic Strains |
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Main Author Ji Soep Shin1 |
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Presenting Author Ji Soep Shin1 |
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Co-Author Nam Soo Han1 |
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Department / Institution / Country Division of Animal, Horticultural, and Food Sciences / Chungbuk National University / Korea, Rep. (대한민국)1 |
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Background and Rationale Ginsenoside Rd is a protopanaxadiol type ginsenoside having tetracyclic terpene sapogenin structure and it is known to exert diverse health promoting activities such as wound healing, immune modulation, anti-obesity, and managed mitochondrial function. |
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Objectives: Indicates the purpose of the study We isolated a lactic acid bacterium from Kimchi showing high β-glucosidase activity and constructed a microbial conversion process to produce ginsenoside Rd from ginsenoside Rb1 by using the isolate. |
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Methodology: Describe pertinent experimental procedures Lactic acid bacteria were isolated from Kimchi and their β-glucosidase activities were tested. Whole cells and lysed cells were used as the crude enzyme in the bioconversion reactions. The lysed cells were prepared by sonication and the bioconversion of Rb1 to ginsenoside Rd was carried out at 30°C for 72 hrs. The strain was identified by phenotypic and genotypic analyses. |
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Results: Summarize the results of the research As result, Leuconostoc mesenteroides EFEL 15 strain was isolated as a potential strain. When the whole cells and lysed cells were reacted with ginsenoside Rb1 in an optimized condition, ginsenoside Rd was produced after 24 hrs. TLC and HPLC analyses showed that the enzyme extract and whole cells hydrolyzed 20-C-β-(1→6)-glucoside linkage of Rb1 compound. |
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Conclusions: State the main conclusions Leuconostoc mesenteroides EFEL 15 strain can be used for overproduction of ginsenoside Rd in the ginseng-derived fermented food. |