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Title Lactobacillus Plantarum ZS2058 Produces Conjugated Linoleic Acid to Ameliorate Colitis |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Influence of Probiotics and Prebiotics on Diet, Nutrition and Drug Metabolism |
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Main Author Wei Chen1 |
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Presenting Author Xiaoming Liu |
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Co-Author Bo Yang Haiqin Chen Xiaoming Liu Hao Zhang |
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Department / Institution / Country School of Food Science and Technology, Jiangnan University / State Key Laboratory of Food Science and Technology / China (中国)1 |
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Background and Rationale CLA has a strong ability to regulate immunity, and this effect has been demonstrated in a great variety of inflammation-related disorders. Lactobacillus plantarum ZS2058 is an efficient producer of conjugated linoleic acid (CLA), in which the mechanism for CLA production was fully characterized. |
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Objectives: Indicates the purpose of the study The aim of the research is to investigate whether CLA producers could show protective effects on colitis through CLA production. |
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Methodology: Describe pertinent experimental procedures Different CLA producers and non-CLA producers were selected. An acute colitis mice model was induced with dextran sodium sulfate (DSS). The mice were treated with different bacteria, CLA, or vehicle. After euthanasia, the inflammatory indexes such as disease activity index (DAI), colon length, colonic histopathologic damage and MPO activity, were investigated. As well as inflammatory cytokines, integrity of colonic mucus layer and CLA concentration were analyzed. |
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Results: Summarize the results of the research Compared to non-CLA producers, L. plantarum ZS2058 significantly alleviated DAI, colon shortening and histological damage. Additionally, L. plantarum ZS2058 could significantly regulates inflammatory cytokine expression in colonic tissue (such as TNF-α, IL-1β, and IL-6, IL-10) as well as protects the colonic mucous layer and epithelium structure. All those results were significantly correlated with CLA production ability. Additionally, colonic CLA concentrations were significantly increased in response to CLA producers treatments, which indicates that CLA producers prevents colitis via generating CLA locally. To further investigatation, the key role of CLA on anti-inflammation, the genetic determinates for CLA production in L. plantarum ZS2058 were knocked-out. Neither intermediates nor CLA was detected in the knock-out mutants. Compared to the wild type, all the mutants practically lost the protective effects on colitis. Meanwhile, those mutants could recover the ability to convert linoleic acid to CLA as well as anti-inflammation when the corresponding gene was completed. |
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Conclusions: State the main conclusions Those current results indicates that lactic acid bacteria with CLA production ability could alleviate colitis via CLA generation. |