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Title Safety Assessment of Lactobacillus Crispatus JDM502 Based on Whole Genome Sequencing
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Probiotic and Prebiotic Safety |
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Main Author Qian Chen1 |
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Presenting Author Qian Chen1 |
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Co-Author Lu Ye1 Chang Liu1 Zhuoyang Zhang2 Xiaokui Guo1 |
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Department / Institution / Country Department of Microbiology and Immunology / Shanghai Jiao Tong University School of Medicine / China (中国)1 Experimental Teaching Center / Shanghai Jiao Tong University School of Medicine / China (中国)2 |
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Background and Rationale As most lactobacilli are non-pathogenic, it is difficult to identify inherent strain properties relevant to safety concerns. The information from conventional experimental methods is restricted to the phenotype. While using whole genome sequencing, some latent safety risks such as antibiotics resistance, virulence factors and harmful metabolites can be revealed. |
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Objectives: Indicates the purpose of the study The objective of this study was to assess the safety of Lactobacillus crispatus JDM 502, a probiotic strain isolated by our group, providing some recommendations for the further application. |
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Methodology: Describe pertinent experimental procedures The whole genome of strain JDM502 was sequenced and the subsequent genes were annotated. Virulence, putative adverse metabolites, and antibiotic resistance related genes, together with genes involved with the genomic stability were identified using BLAST searches. The minimum inhibitory concentration (MIC) of 16 antibiotics to JDM502 were evaluated by E-test. Meanwhile, acute toxicity experiments were performed with C57BL/6 mice which were administered with 108 cfu, 109 cfu, 1010 cfu L. crispatus JDM502 in 200 μL PBS and 200 μL vehicle, respectively, for continuous 7 days. One month later, all the mice were sacrificed, and the pathological injury was measured. |
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Results: Summarize the results of the research The genome of strain JDM502 includes many antibiotic resistance genes, but none of which is at risk of transferring. Most of the 222 virulence related genes are generally safe. Nevertheless, there are 10 genes encoding autolysin, hemolysin B, and invasion associated protein. This strain is non-pathogenic in C57BL/6 mice. |
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Conclusions: State the main conclusions L. crispatus JDM502 is safe and non-pathogenic in C57BL/6 mice. However, there are still potential risks according to the genomic information. When using as probiotics, strain JDM502 is not recommended for those with leaky gut or immune deficiency. |