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Title Identification of Key Proteins and Pathways in Cadmium Tolerance of Lactobacillus Plantarum Strains by Proteomic Analysis |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Detoxification of Environmental Pollutants by Probiotics |
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Main Author Yue Xiao1 |
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Presenting Author Yue Xiao1 |
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Co-Author Qixiao Zhai1 2 Jianxin Zhao1 Fengwei Tian1 2 Hao Zhang1 2 Arjan Narbad2 3 Wei Chen1 2 4 |
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Department / Institution / Country State Key Laboratory of Food Science and Technology, School of Food Science and Technology / Jiangnan University / China (中国)1 UK-China Joint Centre on Probiotic Bacteria / UK-China Joint Centre on Probiotic Bacteria / United Kingdom2 Gut Health and Food Safety Programme / Institute of Food Research / United Kingdom3 Beijing Innovation Centre of Food Nutrition and Human Health / Beijing Technology & Business University / China (中国)4 |
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Background and Rationale Our previous study confirmed the protective potential of Lactobacillus plantarum (L. plantarum) strains in alleviation of cadmium (Cd) toxicity in vivo and demonstrated that the observed protection largely depended on the tolerance of the strains to Cd-induced stress. It was also observed that there were significant intra-species differences in Cd tolerance of L. plantarum strains. |
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Objectives: Indicates the purpose of the study The objective of the present study was to investigate the Cd tolerance mechanisms of L. plantarum strains based comparative proteomics. |
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Methodology: Describe pertinent experimental procedures The Cd-tolerance related key proteins and pathways within the L. plantarum species were investigated by using iTRAQ based proteomic approach. L. plantarum strains CCFM8610 (strongly resistant to Cd) and CCFM191 (sensitive to Cd) were selected for comparative proteomic analysis. The proteomic results were further confirmed by RT-qPCR and by the measurement of several biological properties of the bacterial cells in response to Cd exposure. |
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Results: Summarize the results of the research Of the total 1415 identified proteins, 206 were differentially expressed for the comparison of natural proteomic profiles of CCFM8610 and CCFM191, 27 were differently regulated in CCFM8610 after Cd exposure, and 111 were changed in CCFM191 in response to Cd stress. Both strains showed physiological alterations in energy metabolism, purine and pyrimidine metabolism, global stress responses, lipid and amino acid metabolism, metal binding properties, cell wall biosynthesis and transporters in response to Cd exposure. |
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Conclusions: State the main conclusions We propose that the underlying mechanism of the exceptional Cd tolerance of CCFM8610 may be attributed to the following: (a) a specific energy-conservation survival mode; (b) mild induction of its cellular defense and repair system; (c) an enhanced biosynthesis of hydrophobic amino acids in response to Cd;(d) inherent superior Cd binding ability and effective cell wall biosynthesis ability; (e) a tight regulation on ion transport; (f) several key proteins, including prophage P2b protein 18, CadA, mntA and lp_3327. |