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Title Gut Microbiota Mediates Islet Cell Injury Induced by Low-dose DSS |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Translational Microbiome Studies |
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Main Author Xin Yang1 |
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Presenting Author Xin Yang1 Chenhong Zhang1 Liping Zhao1 |
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Co-Author Chenhong Zhang1 Liping Zhao1 |
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Department / Institution / Country Shanghai Jiao Tong University / School of Life Sciences and Biotechnology / China (中国)1 |
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Background and Rationale Emerging evidence showed that microbes may be involved in immune response and antigen presentation during induction and continued persistence. Our study found that low-dose dextran sulfate sodium (DSS) can induce type 1 diabetes (T1D) in mice, but the mechanism is not clear. |
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Objectives: Indicates the purpose of the study Trying to clarify the mechanism by which gut microbiota mediates islet injury in T1D. |
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Methodology: Describe pertinent experimental procedures Low-dose DSS was used as an oral treatment for C57BL male mice for 3 months. Drinking water, diet and body weight were measured every 3 days and daily faeces were also collected. V3-V4 region in 16s rRNA were sequenced to analyze the structure of gut microbiota. We also tested blood lipids, liver fat, blood glucose, blood insulin and injury of islet. Then we use broad spectrum antibiotics (ABX) to deplete commensal gut microbiota and the effects of low-dose DSS on glycometabolism were observed. |
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Results: Summarize the results of the research The mice with low-dose DSS treatment showed a frequent urination, increased thirst and increased hunger phenomenons, without increasing net energy intake since the experiment started. After 8 weeks with low-dose DSS, the mice had abnormal glucometabolism, impaired pancreas, and insufficient insulin secretion. The structure of the gut microbiota also showed significantly shift during the DSS treatment. Moreover, DSS did not induce abnormal glycometabolism in the mice with broad spectrum antibiotic treated. |
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Conclusions: State the main conclusions The study suggested that gut microbiota mediates islet cell injury induced by low-dose DSS. |