|
Title Effect of Domestication on the Intestinal Microbiome of Asturcon Horses. |
|
Type Poster Presentation |
|
Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
|
Topic Translational Microbiome Studies |
|
Main Author David Ríos-Covián2 |
|
Presenting Author Carlos Gómez-Gallego1 |
|
Co-Author Li Ang3 Carlos Gómez-Gallego1 Amparo García4 Clara G. de los Reyes-Gavilán2 Seppo Salminen1 Miguel Gueimonde2 |
|
Department / Institution / Country Functional Foods Forum / University of Turku / Finland (Suomi)1 Department of Microbiology and Biochemistry of Dairy Products / Instituto de Productos Lácteos de Asturias (IPLA-CSIC) / Spain (España)2 Precision Medicien Centrer / Hospital of Zhengzhou University / China (中国)3 - / Asociación Pura Raza Asturcón / Spain (España)4 |
|
Background and Rationale The Asturcon horse is an old horse breed belonging to the Celtic ponies group. Domestic horses are more sensitive to gastrointestinal diseases than wild/feral animals, and the Asturcon breed is no exception. In comparison with modern horse breeds, the Asturcon provides a model in which wild and domesticated populations co-exist over a uniform genetic background. The ancient microbiota may constitute a microbial reference for risk reduction in domestic horses as well as a source for the isolation of specific strains or combinations aimed at enhancing horses’ health. |
|
Objectives: Indicates the purpose of the study Our aim was to compare the microbiome of wild and domestic Asturcons and isolate strains as potential horse probiotics. |
|
Methodology: Describe pertinent experimental procedures Fresh fecal samples from nine Asturcon mares (three wild and 6 domesticated were collected). Samples were plated in MRS for isolation of lactobacilli and submitted to microbiome analyses and SCFA determination |
|
Results: Summarize the results of the research Wild-horses’ showed several microbiota differences, form kingdom to species level, when compared with domesticated animals. The wild Asturcons presented higher levels of Puccinia striformis, Penicillium sclerotiorum, Lawsonia intracellularis, Eubacterium halii, Bacteroides salanitronis, Christensenella masilensis, Treponema azotonutricium, Lachnoclostridium phytofermentans, Streptococcus anginosus, Streptococcus suis, Lactobacillus plantarum, Staphyloccocus aureus and Campylobacter. Domesticated horses presented higher levels of different rhizosphere and plant-symbiotic microorganisms, including Sphingomonas taxi, Sphingomonas panacis, Dietzia timorensis, Stenotomonas rhizophila, Pseudomonas rhizospherae, Bradyrhizobium, Agrobaterium tumefaciens, Fusarium graminearum, Rhodococcus fascians, Tiletia indicum, Actinomyces naeslundii, Methylobacterium zatmanii and Clostridium cellulovorans. The Lactobacillus strains isolated belonged to the species Lactobacillus equi, Lactobacillus equigenerosi or Lactobacillus hayakitensis. Wild animals presented, in general, higher levels of bacteriophages than their domestic counterparts, specially of lactobacillus-phages, and higher levels of SCFA. |
|
Conclusions: State the main conclusions Our results underline the impact of domestication on the horse microbiome. The isolates of L. equi, L. equigenerosi and L. hayakitensis obtained here constitute a potential source for the development of probiotics for horses. |