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Title Role of Human-Residential Bifidobacteria in the Degradation of Food-Derived Opioid Peptides |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Influence of Probiotics and Prebiotics on Diet, Nutrition and Drug Metabolism |
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Main Author Chyn Boon Wong1 |
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Presenting Author Chyn Boon Wong1 |
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Co-Author Takuma Sakurai1 Toshitaka Odamaki1 Jin-zhong Xiao 1 |
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Department / Institution / Country Next Generation Science Institute / Morinaga Milk Industry Co., Ltd. / Japan (日本)1 |
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Background and Rationale Food-derived opioid peptides are known as bioactive peptides that can interact with opioid receptors and possess beneficial health effects. However, recent epidemiological evidence has identified some food-derived opioid peptides as potential risk factors for human diseases such as mental disorders, celiac disease, and sudden infant death syndrome. Bifidobacteria, which are the major member of human gut microbiota, particularly in infant gut, have increasingly been shown to exert positive health benefits to host. Bifidobacteria naturally inhabit a range of ecological niches and can be classified into two major groups; bifidobacterial species of human origins as human-residential bifidobacteria (HRB) while other species which are the natural inhabitants of animals or environment as non-HRB. |
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Objectives: Indicates the purpose of the study Here we evaluate the potential role of bifidobacteria in the degradation of food-derived opioid peptides. |
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Methodology: Describe pertinent experimental procedures We tested the ability of 18 bifidobacterial strains, representing nine infant-type human-residential bifidobacteria (HRB), four adult-type HRB, and five non-HRB, to degrade human milk- and bovine milk-derived casomorphin-7, and wheat gluten-derived gliadorphin-7. Dipeptidyl peptidase (DPP) activity of bifidobacteria was determined using fluorescence assay and the final contents of opioid peptides upon incubation were quantified using LC-MS/MS. |
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Results: Summarize the results of the research Our findings reveal an origin-dependent degradative capability of bifidobacteria in food-derived opioid peptides whereby infant-type HRB strains showed a relatively higher DPP activity than non-HRB strains. For instance, strains of Bifidobacterium longum subsp. infantis and B. bifidum effectively hydrolysed all three opioid peptides. We further evaluated the degradative capabilities of 29 strains of B. bifidum and selected the strains with higher potential in degrading opioid peptides. |
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Conclusions: State the main conclusions These results suggest the possible role of infant-type HRB in infant gut to eliminate food-derived opioid peptides, protect against chronic diseases and contribute to infant health development. Selected strains of B. bifidum with high degradative capabilities could be used as novel probiotic interventions to aid in host metabolism and improve human health. |