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Title Lactic Acid Bacteria Growth Promotion of Xylooligosaccharides Derived From Alkali-Pretreated and Acid-Debranched Rice Husk |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Development of Probiotic and Prebiotic Foods, Medical Foods, Supplements and Drugs |
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Main Author Wannaporn Klangpetch1 |
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Presenting Author Wannaporn Klangpetch1 |
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Co-Author |
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Department / Institution / Country Agro-Industry / Naresuan University / Thailand (ไทย)1 |
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Background and Rationale Rice husk is the rice milling industrial by-product gained in a huge amount. It is rich in lignocelluloses, previously found at 22% of cellulose content and 23% of hemicellulose content. These lignocellulosic materials have been used widely for oligosaccharide productions. |
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Objectives: Indicates the purpose of the study In this study, we investigated the effects of alkali pretreatment and acid debranching on changing of arabinoxylan and arabinose/xylose ratio including the ability of commercial xylanases to produce xylooligosaccharides rice husk. The lactic acid bacteria growth promotion of the oligosaccharides was also observed. |
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Methodology: Describe pertinent experimental procedures Firstly, rice husk was treated with 2% NaOH and diluted formic acid. The total sugar contents of the alkali-pretreated and acid- debranched rice husk was measured. At the same time, the xylose, arabinose, glucose, galactose and mannose content, the arabinose/xylose ratio (A/X) and arabinoxylan contents were also determined by HPLC analysis. Then, the commercial xylanases were used to produce oligosaccharides (pH 6, 50°C, 4 h). Thin Layer Chromatography and High Performance Anion Exchange chromatography - Pulsed Amperometric Detection were performed to confirm the oligosaccharides profile of the products. The hydrolysate was used as a single carbon source for evaluation of the growth promotion effect on lactic acid bacteria. |
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Results: Summarize the results of the research The yields of alkali-pretreated rice husk were decreased to 68.7%, while the total sugar content was increased from 19.9% to 28.0%. The total sugar content of acid-debranched rice husk was also increased up to 4%. Besides, the alkali pretreatment decreased A/X ratio from 0.14 to 0.13 whereas the arabinoxylan content was increased from 11.5% to 18.6%. Distinguishably, xylobiose was produced by using commercial endoxylanases. The hydrolysate could promote the growth of Lactobacilli and Bifidobacteiria. |
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Conclusions: State the main conclusions This study has proved the possibility of using rice husk as a source of oligosaccharide production. |