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Title Gut Microbiota of Healthy Young Thai Children Consuming Synbiotics Supplemented Formula |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Probiotic and Prebiotic Clinical Research, From Newborns to Elderly |
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Main Author Charmaine Chew1 |
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Presenting Author Charmaine Chew1 |
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Co-Author Christophe Lay1 Sebastian Tims2 Pantipa Chatchatee3 Mongkol Lao-Araya4 Suwannee Uthaisangsook5 Pensri Kosuwon6 Jan Knol2 |
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Department / Institution / Country Research & Innovation / Danone Nutricia Research / Singapore1 Research & Innovation / Danone Nutricia Research / Netherlands2 Allergy & Immunology / King Chulalongkom Memorial Hospital / Thailand (ไทย)3 Pediatric Allergy and Immunology / Chiang Mai University Hospital / Thailand (ไทย)4 Pediatrics / Naresuan University / Thailand (ไทย)5 Pediatrics / Sringarind Hospital & Khon Kaen University / Thailand (ไทย)6 |
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Background and Rationale Young children (1-3 years old) undergo a progressive transition of diet beginning with introduction of weaning food. Diet plays a role to provide macro & micro-nutrients required for their growth and have an impact on microbiota composition. The gut microbiota of young children is presumed to be adult-like. However, there is limited knowledge and reports of microbiota composition at this age especially in Asian countries.
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Objectives: Indicates the purpose of the study To understand the influence of young child formula supplemented with synbiotics short-chain galacto-oligosaccharides (scGOS) and long-chain fructo-oligosaccharides (lcFOS) with Bifidobacterium breve M-16V on the gut microbiota of 1-3 years old Thai young children. |
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Methodology: Describe pertinent experimental procedures From a randomized, double-blind, placebo-controlled, multi-centre, 12 week clinical study in Thailand, fecal samples were collected from subjects at baseline, week 6 and week 12. The intervention consisted of young child formula (YCF) supplemented with specific mixture of prebiotics (1g/100mL scGOS/lcFOS 9:1 ratio) and 1.8 x 107 CFU/g of Bifidobacterium breve M-16V (n=65), or control formula (n=64). Quantitative real-time PCR (qPCR) and 454 pyrosequencing of V3-V5 16S rRNA gene were performed to provide insights into the microbiota community over time.
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Results: Summarize the results of the research This study confirmed a high prevalence of the Bifidobacterium genus (20.7% average) in young children at all time points regardless of intervention – composing of B. breve, B. longum, B. bifidum, B. catenulatum and B. adolescentis. We also demonstrated a bifidogenic effect in the intervention group, and observed that the synbiotics supplementation also did not diminish endogenous B. breve. 16S rRNA sequencing revealed that toddler microbiota is not only dominated by Firmicutes but also Actinobacteria which was presumed to dwindle in childhood.
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Conclusions: State the main conclusions YCF supplemented with a synbiotics mixture significantly increased fecal bifidobacteria level in healthy young children aged 1-3 years. However, the influence of bifidobacteria on other beneficial bacteria in this microbial ecosystem has to be further investigated. |