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Title Autoinducer-2 Quorum Signaling in Probiotics: A Mechanism of Gut Microbiome Modulation |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Translational Microbiome Studies |
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Main Author Hyunjoon Park1 2 |
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Presenting Author Hyunjoon Park1 2 |
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Co-Author Kyuyeon Lee1 Eunsung Jung4 Soyoung Yeo3 Seokwon Kang2 Choonghwan Lee4 Wilhelm Holzapfel1 |
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Department / Institution / Country Advanced Green Energy and Environment / Handong Global University / Korea, Rep. (대한민국)1 Life Science / Handong Global University / Korea, Rep. (대한민국)2 Green-Bio Science and Technology / Seoul National University / Korea, Rep. (대한민국)3 Bioscience and Biotechnology / Konkuk University / Korea, Rep. (대한민국)4 |
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Background and Rationale Bacterial symbioses are essential in the human host. Especially, gut microbiota is reported that closely related to physiological traits and diseases. Therefore, the gut microbiota modulation by probiotics as bacterial therapeutics are being focused, and many of studies are supporting the concept. However, the mechanism of gut microbiota modulation by probiotics administration is not revealed obviously. Bacterial quorum sensing is common events in the bacterial community. In particular, LuxS-mediated autoinducer-2 (AI-2) signaling system is found in a wide variety of Gram-positive and Gram-negative bacteria, including some of probiotics lactic acid bacteria species. It has been reported to regulate corresponding gene expression of adaptation and resistance to the environment. Furthermore, recent studies suggested that AI-2 activity can assist to restore the balance of microbiota in antibiotic-induced dysbiosis and the response of the epithelial innate immune system. |
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Objectives: Indicates the purpose of the study Therefore, we expect that AI-2 signaling status may a cause for the modulation of the gut microbiome. |
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Methodology: Describe pertinent experimental procedures The bacterial community of each sample was analyzed using qRT-PCR, Illumina Mi-Seq through the 16S rRNA gene. Mouse host and bacterial gene expression were profiled by Illumina Hi-Seq 2000 RNA sequencing, and its untargeted metabolites were analyzed by GC-TOF-MS. ILC3 cells changes observed by BD Aria FACS system |
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Results: Summarize the results of the research AI-2 molecules changed the composition ratio of Firmicutes/Bacteroidetes compared to the control. Mouse gut metabolites were also partially modulated by AI-2 and QSI treatment in the organic acids, nucleic acids, and amino acids. But the correlation between metabolite production and mRNA expression was not observed. |
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Conclusions: State the main conclusions Although the AI-2 signaling in gut bacteria are still insufficiently understood, the results indicate that the AI-2 signaling property of probiotics may a key mechanism cause the gut microbiome modulation. |