|
Title Characterization of Purine Degrading Lactic Acid Bacteria and Evaluation of the Serum Uric Acid Lowering Effect in Hyperuricemic Rats |
|
Type Poster Presentation |
|
Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
|
Topic Development of Probiotic and Prebiotic Foods, Medical Foods, Supplements and Drugs |
|
Main Author Hsieh-Hsun Ho1 Pei-Shan Hsieh1 Yi-Wei Kuo1 |
|
Presenting Author Hsieh-Hsun Ho1 Pei-Shan Hsieh1 |
|
Co-Author V. An-Erl King2 |
|
Department / Institution / Country Research and Development Dept. / glac Biotech Co., Ltd / Taiwan (台灣)1 Department of Food Science and Biotechnology / National Chung Hsing University / Taiwan (台灣)2 |
|
Background and Rationale Uric acid is the final product of purine metabolization in liver, and it was excreted with urine by the kidney. When the uric acid was excessive in the body, it caused high serum uric acid (hyperuricemia). In the evolution, humans and some primates lose of uricase gene (allantoin) that play the important role of uric acid metabolization, and the refined food culture promoted the prevalence rate of hyperuricemia more dramatic. Hyperuricemia was caused by abnormally high level of uric acid in the blood which results in the gout symptom. It had also been recognized as a risk factor for chronic kidney disease. Lactic acid bacteria, such as lactobacilli, produce lactic acid as a major metabolic end product of carbohydrate fermentation. Oral intake of Lactobacillus may have beneficial effects for the host, by, for example, activating immune responses. but few reports have investigated the effect of Lactobacillus ingestion on hyperuricemia. |
|
Objectives: Indicates the purpose of the study Hyperuricemia usually was cured by treated with drug, for example allopurinol, but drug treatment usually caused strong side effects. Therefore, we want to explore whether Hyperuricemia could be treated by supplementing specific lactic acid bacteria. |
|
Methodology: Describe pertinent experimental procedures Inosine and guanosine were cultured with lactic acid bacteria. The cultured solution were filtered and injected into a HPLC device to analysis the concentration of inosine and guanosine. These purine degrading lactic acid bacteria isolated by HPLC methods were further to use in animal test. |
|
Results: Summarize the results of the research The in vitro results, we isolated four Lactobacillus spp. (L. fermentum, L. acidophilus, L. reuteri and L. plantarum) that had the ability to degrade inosine and guanosine. Moreover, the L. reuteri and L. fermentum not only had the high purine nucleosides degrading ability, they also could decrease the serum uric acid level in hyperuricemia animal model. |
|
Conclusions: State the main conclusions In our study, we found out two strains of lactic acid bacteria which could degrade purine nucleosides and reduce the concentration of uric acid in mice serum. The finding suggest that, Hyperuricemia could be treated by supplementing these lactic acid bacteria. |