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Title Lactic Acid Bacteria Regulates Blood Sugar by Metabolizing Carbohydrates and Regulating the Gene Expression of Intestinal Cells |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Development of Probiotic and Prebiotic Foods, Medical Foods, Supplements and Drugs |
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Main Author Dr Hsieh-Hsun Ho1 Zun-Jie Syu1 Pei-Shan Hsieh1 Dr Yi-wei Kuo |
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Presenting Author Pei-Shan Hsieh1 Dr Yi-wei Kuo |
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Co-Author V. An-Erl King2 |
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Department / Institution / Country Research and Development Dept. / glac Biotech Co., Ltd / Taiwan (台灣)1 Department of Food Science and Biotechnology / National Chung Hsing University / Taiwan (台灣)2 |
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Background and Rationale In recent years, due to diet and lifestyle changes, metabolic syndrome had become more and more common disease. In which, high blood glucose may cause diabetes. Diabetes mellitus type 2 (also known as type 2 diabetes) is a long-term metabolic disorder that is characterized by high blood sugar, insulin resistance, and relative lack of insulin. T2DM may be associated with some complications, including cardiovascular disease, stroke, blindness, kidney failure or Alzheimer's disease. Rates of T2DM have increased markedly since 1960 in parallel with obesity. As of 2015 there were approximately 392 million people diagnosed with the disease compared to around 30 million in 1985. Typically it begins in middle or older age, although rates of T2DM are increasing in young people. In addition, some probiotics effectively ameliorated T2DM by improving hyperglycemia, glucose tolerance. The antidiabetic effect of Lactobacillus is increasingly recognized worldwide.
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Objectives: Indicates the purpose of the study It was known that lactic acid bacteria had the functions of maintaining the intestinal environment, anti-inflammation, anti-allergy, and we want to findout the special strain of lactic acid bacteria who could consumptied sugar efficiently in gut. |
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Methodology: Describe pertinent experimental procedures There were 25 strains of lactic acid bacteria was evaluated by the ability of metabolizing carbohydrates in MRS broth with 2% and 6% monosaccharide. These 4 × 108 lactic acid bacteria were co-cultured with 4 × 105 Caco-2 cells for 20 hours. We measured the gene expression of the glucose transporter gene and the sodium glucose co-transporter gene on different lactic acid bacteria and Caco-2 cell. In the animal experiment, the db/db mice were feeded by lactic acid bacteria for a month to observe the change of blood sugar. |
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Results: Summarize the results of the research The in vitro test, we screened few strains lactic acid bacteria by the ability of metabolizing carbohydrates from 25 strains, and 4 strains showed the best metabolic ability, including Lactobacillus reuteri GL-104, Lactobacillus salivarius AP-32, Lactobacillus acidophilus TYCA06 and Lactobacillus gasseri MH-68. The intestinal cells co-culture experiment showed the 4 strains of Lactobacillius had different regulatory effects on the glucose transporter (GLUT) gene but also the sodium glucose co-transporter (SGLT) gene, respectively.
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Conclusions: State the main conclusions However, the Lactobacillus reuteri GL-104 and Lactobacillus salivarius AP-32 strains ware superior in sugars metabolizing, and the regulation of intestinal cell glucose transporter gene expression was different from other strains. We suggested that GL-104 and AP-32 strains have the potential of regulating blood sugar and could apply on diabetes treatment in the future. |